Similarity of salivary microbiome in parents and adult children

, , , , ,
1 Faculty of Medicine and Health Technology and Finnish Cardiovascular Research Center, University of Tampere, Tampere, Finland
2 Fimlab laboratories ltd., Tampere, Finland
3 Department of Otorhinolaryngology, Faculty of Medicine and Health Technology, University of Tampere, Tampere, Finland
4 Department of Oral and Maxillofacial diseases, Tampere University Hospital, Tampere, Finland
5 Oral Health Services, City of Tampere, Tampere, Finland
6 Department of Molecule Microbiology, Faculty of Medicine and Health Technology, University of Tampere, Tampere, Finland
DOI
10.7287/peerj.preprints.27565v1
Published
Accepted
Subject Areas
Bioinformatics, Genetics, Microbiology
Keywords
16S rRBA gene, metagenomics, Next Generation Sequencing, Saliva, Microbiota, Similarity
Copyright
© 2019 Sundström et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Sundström K, Mishra PP, Pyysalo MJ, Lehtimäki T, Karhunen PJ, Pessi T. 2019. Similarity of salivary microbiome in parents and adult children. PeerJ Preprints 7:e27565v1

Abstract

Background: Human saliva contains approximately 700 bacterial species but the relatedness of salivary bacteria from parents to adult children is not investigated in humans. The objectives were to investigate the entirety of salivary bacterial DNA profiles and whether and how families share these profiles and also compare these communities between adult parent-off-spring pairs using 16S rRNA gene amplicon sequencing.

Results: The most abundant phyla in two separate families were Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria and Actinobacteria. Family ties explained 13 % of the variance between individuals’ bacterial communities (R2=0.13; P=0.001). Mothers shared more OTUs with their adult children compared to fathers, but this linkage seemed to be weaker in the family with older adult children. We identified 29 differentially abundant genus level OTUs (FDR < 0.05) between the families, which accounted for 31 % of the total identified genus level OTUs

Conclusions: Our results indicate that adult family members share bacterial communities and adult children were more similar to mothers than fathers. Our results suggest implicitly that a similarity in oral microbiome between parent-child pairs is present, but may change over time.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Bacterial taxa with adjusted P-values obtained from SILVA. P-values are adjusted for multiple testing correction in order to reduce false positive results

Bacterial taxa with adjusted P-values obtained from Human Oral Microbiome Database (HOMD). P-values are adjusted for multiple testing correction in order to reduce false positive results

DOI: 10.7287/peerj.preprints.27565v1/supp-1

Venn diagrams showing OTU overlaps between family members

DOI: 10.7287/peerj.preprints.27565v1/supp-2

Venn diagrams showing OTU overlaps between family members

DOI: 10.7287/peerj.preprints.27565v1/supp-3

Venn diagrams showing OTU overlaps between family members

DOI: 10.7287/peerj.preprints.27565v1/supp-4