Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using Tobacco rattle virus
- Published
- Accepted
- Subject Areas
- Molecular Biology, Plant Science
- Keywords
- Hibiscus hamabo Sieb. et Zucc., Tobacco rattle virus vector, Virus-induced gene silencing, Cloroplastos alterados 1, Gene silencing
- Copyright
- © 2019 Wang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using Tobacco rattle virus. PeerJ Preprints 7:e27541v1 https://doi.org/10.7287/peerj.preprints.27541v1
Abstract
Background. Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. Because the genetic transformation of H. hamabo is difficult, molecular breeding and gene functional studies have been severely restricted. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods. In this study, we tested the efficiency of a Tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfiltration. Results. The leaves of H. hamabo showed white streaks typical of CLA1 gene silencing three weeks after agroinfiltration. In agroinfiltrated H. hamabo plants, the CLA1 expression levels in leaves with white streaks were all significantly lower than in those of mock-infected and control plants. Conclusions. Thus, this virus-induced gene silencing system was efficient in H. hamabo and may be a powerful tool for large-scale reverse-genetic analyses of gene functions in H. hamabo.
Author Comment
This is a submission to PeerJ for review.