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Distinct gene expression and secondary metabolite profiles for suboptimal mycorrhizal colonization in wild-type and the jasmonic acid deficient spr2 tomato mutant

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PeerJ Preprints Distinct gene expression and secondary metabolite profiles for suboptimal mycorrhizal colonization in wild-type and the jasmonic acid deficient spr2 tomato mutant https://t.co/nCfTZkEbVV
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Supplemental Information

Untargeted principal components analysis on metabolic fingerprinting of mycorrhizal tomato roots

Data were generated from control (CTR) and mycorrhizal (MYC) conditions for the experiment yielding “low” and “high” AMF colonization levels.

DOI: 10.7287/peerj.preprints.27533v1/supp-1

Metabolic heat-map analysis

Data were generated with the 100 most intense ions detected in tomato roots under control (CTR) and mycorrhizal (MYC) conditions for the experiment yielding “low” and “high” AMF colonization levels.

DOI: 10.7287/peerj.preprints.27533v1/supp-2

qPCR analysis

Oligonucleotides employed for qPCR analysis of AMF colonization related genes.

DOI: 10.7287/peerj.preprints.27533v1/supp-3

Metabolomic analysis

Metabolites whose JA-dependent abundance correlated with AMF colonization efficiency.

DOI: 10.7287/peerj.preprints.27533v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Kena Casarrubias-Castillo conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Josaphat M Montero-Vargas conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Nicole Dabdoub-González performed the experiments, analyzed the data, prepared figures and/or tables.

Robert Winkler analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper.

Norma A Martinez-Gallardo performed the experiments, analyzed the data, prepared figures and/or tables.

Hamlet Aviles-Arnaut conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

John P Délano-Frier conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

1) Zenodo

2) 10.5281/zenodo.2550944, 10.5281/zenodo.2554756

3) https://doi.org/10.5281/zenodo.2550944, https://doi.org/10.5281/zenodo.2554756

Funding

NDG (No. 191369), JMMV (No. 191369) and KCC (No. 227935) were supported by postgraduate scholarships granted by The National Council of Science and Technology (Conacyt, México). HAA was supported by a Basic Science grant conceded by Conacyt (No. 239695). The laboratory for biochemical and instrumental analysis (RW) was funded by the CONACyT Fronteras project 2015-2/814 and the bilateral grant Conacyt-DFG 2016/277850. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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