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MCM2 partial silencing inhibits apical dominance and promotes chlorophyll pigmentation in tomato

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MCM2 partial silencing inhibits apical dominance and promotes chlorophyll pigmentation in tomato https://t.co/cjXbR62Mus Background: We have previously combined loss- and gain-of-function approaches to investigate the function of Arabidopsis minichromosome maintenanc…
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Supplemental Information

Up-regulated genes in LeMCM2 silenced lines

Up-regulated genes in LeMCM2 silenced lines compare to wild-type plants

DOI: 10.7287/peerj.preprints.27513v1/supp-1

Down-regulated genes in LeMCM2 silenced lines

Down-regulated genes in LeMCM2 silenced lines compare to wild-type plants

DOI: 10.7287/peerj.preprints.27513v1/supp-2

Primer sequences

Primer sequences used for generating binary vector and detecting transgenic plants

DOI: 10.7287/peerj.preprints.27513v1/supp-3

Composition of lysate for p loidylevel detection

Composition of lysate for p loidy level detection

DOI: 10.7287/peerj.preprints.27513v1/supp-4

Composition of lysate for p loidylevel detection

The Arabidopsis MCM2 protein sequence was used to search for tomato MCM2 protein via Sol Genome Network ( https://solgenomics.net/tools/blast/ ) .

DOI: 10.7287/peerj.preprints.27513v1/supp-5

The tomato MCM2 specific cDNA sequence

The specific cDNA sequence for generating MCM2-RNAi vector

DOI: 10.7287/peerj.preprints.27513v1/supp-6

Seed size from the LeMCM2-silenced plants decreased atmaturity.

Seeds from transgenic plants (a) and wild-type plants (b)

DOI: 10.7287/peerj.preprints.27513v1/supp-7

Fraction of ploidylevel of Ln-MCM2-silenced tomatoplants (line 13) and wild-type control plants.

Samples were fully expanded leaves (a) and young leaves (b).

DOI: 10.7287/peerj.preprints.27513v1/supp-8

tomato DNAchip raw data (CK)

tomato DNAchip raw data (CK)

DOI: 10.7287/peerj.preprints.27513v1/supp-9

tomato DNAchip raw data (RNAi line 10)

tomato DNAchip raw data (RNAi line 10)

DOI: 10.7287/peerj.preprints.27513v1/supp-10

tomato DNAchip raw data (RNAi line 11)

tomato DNAchip raw data (RNAi line 11)

DOI: 10.7287/peerj.preprints.27513v1/supp-11

tomato DNAchip raw data (RNAi line 13)

tomato DNAchip raw data (RNAi line 13)

DOI: 10.7287/peerj.preprints.27513v1/supp-12

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ma-ye Gao performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Jin Liang performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Hai-ting Liu performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Di-an Ni conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

The name of the database :annotare

The accession/id number:E-MTAB-7677

https://www.ebi.ac.uk/fg/annotare/

Data Deposition

The following information was supplied regarding data availability:

The name of the database :annotare

The accession/id number:E-MTAB-7677

https://www.ebi.ac.uk/fg/annotare/

Funding

This work was funded by grants from the Innovation Program of Shanghai Municipal Education Commission (13YZ122). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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