MCM2 partial silencing inhibits apical dominance and promotes chlorophyll pigmentation in tomato
- Published
- Accepted
- Subject Areas
- Agricultural Science, Biotechnology, Cell Biology, Plant Science
- Keywords
- Tomato MCM2, DNA replication, Apical dominance, Chlorophyll pigmentation
- Copyright
- © 2019 Gao et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. MCM2 partial silencing inhibits apical dominance and promotes chlorophyll pigmentation in tomato. PeerJ Preprints 7:e27513v1 https://doi.org/10.7287/peerj.preprints.27513v1
Abstract
Background: We have previously combined loss- and gain-of-function approaches to investigate the function of Arabidopsis minichromosome maintenance (MCM) protein subunit 2 (MCM2). However MCM2 loss of function leads to the death of embryos, thus precluding the analysis of homozygous MCM2 mutants.
Methods: To further understand the function of MCM2, we generated transgenic tomato plants via RNA interference silencing. And microarray slide hybridization was used to compare gene expression level between LeMCM2 -silenced lines and wild-type plants.
Results: We obtained 4 LeMCM2 -silenced lines. The expression level in LeMCM2 -silenced lines was 20-80% of that of wild-type. And LeMCM2 -silenced lines display loss of apical dominance, enhanced chlorophyll accumulation and reduced leaf cell endoduplication. In addition, the expression levels of genes involved in DNA replication was altered in LeMCM2 -silenced lines significantly.
Conclusion: In general, the data described here provided additional evidence for the relationship between genes involved in DNA replication and apical dominance and chlorophyll pigmentation.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Up-regulated genes in LeMCM2 silenced lines
Up-regulated genes in LeMCM2 silenced lines compare to wild-type plants
Down-regulated genes in LeMCM2 silenced lines
Down-regulated genes in LeMCM2 silenced lines compare to wild-type plants
Primer sequences
Primer sequences used for generating binary vector and detecting transgenic plants
Composition of lysate for p loidylevel detection
Composition of lysate for p loidy level detection
Composition of lysate for p loidylevel detection
The Arabidopsis MCM2 protein sequence was used to search for tomato MCM2 protein via Sol Genome Network ( https://solgenomics.net/tools/blast/ ) .
The tomato MCM2 specific cDNA sequence
The specific cDNA sequence for generating MCM2-RNAi vector
Seed size from the LeMCM2-silenced plants decreased atmaturity.
Seeds from transgenic plants (a) and wild-type plants (b)
Fraction of ploidylevel of Ln-MCM2-silenced tomatoplants (line 13) and wild-type control plants.
Samples were fully expanded leaves (a) and young leaves (b).
tomato DNAchip raw data (CK)
tomato DNAchip raw data (CK)
tomato DNAchip raw data (RNAi line 10)
tomato DNAchip raw data (RNAi line 10)
tomato DNAchip raw data (RNAi line 11)
tomato DNAchip raw data (RNAi line 11)
tomato DNAchip raw data (RNAi line 13)
tomato DNAchip raw data (RNAi line 13)