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Evolutionarily conserved roles of Caenorhabditis elegans perilipin in lipolysis

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Evolutionarily conserved roles of Caenorhabditis elegans perilipin in lipolysis https://t.co/kPYBLo9Sn2 https://t.co/iwgrfIcCjo
290 days ago
Evolutionarily conserved roles of Caenorhabditis elegans perilipin in lipolysis https://t.co/1HtjEMmNOs https://t.co/MYfBXl10Lq
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Supplemental Information

Cloning strategy overview for the construction of a rescue plasmid used in CAS9/CRISPR-dependent mKate2 insertion next to the native locus of PLIN-1

DOI: 10.7287/peerj.preprints.27467v1/supp-2

Raw data of experiments represented in Fig. 3-5

(Sheet 1) Animal counting in the experiment represented in Fig. 3. (Sheet 2) Animal counting in the experiments represented in Fig. 5. (Sheet 3) Object analysis in the images, represented in Fig. 4.

DOI: 10.7287/peerj.preprints.27467v1/supp-3

Raw images used in object analysis represented in Fig. 4

(Supplementary Figure S4-S6) Individual analysed 8-bit TIFF 5µm z-stack images cropped to the analysed extent of the first embryos in the control line RD204. (Supplementary Figure S7-S9) Individual analysed 8-bit TIFF 5µm z-stack images cropped to the analysed extent of the first embryos in the experimental line KV2.

DOI: 10.7287/peerj.preprints.27467v1/supp-4

Additional Information

Competing Interests

Marta Kostrouchová is an Academic Editor for PeerJ. The authors declare there are no competing interests.

Author Contributions

Filip Kaššák conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Ahmed A Chughtai conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Marta Kostrouchová conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Supplementary Table S2 lists numbers and proportions of counted animals for experiments represented in Fig. 3 and 5 and the raw data of object analysis in Fig. 4. Supplementary Data S4 contains Supplementary Figures S4-S9 representing raw unadjusted 8-bit TIFF images of first identified embryos cropped to the extent used for image analysis. Supplementary Fig. S4-S6 and S7-S9 represent images of RD204 and KV2 lines respectively.

Funding

This work was supported by the European Regional Development Fund “BIOCEV—Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec” (CZ.1.05/1.1.00/02.0109) (The Start-Up Grant to the group Structure and Function of Cells in Their Normal State and in Pathology—Integrative Biology and Pathology (5.1.10)) and the LQ1604 National Sustainability Program II (Project BIOCEV-FAR) and the project Biocev (CZ.1.05/1.1.00/02.0109) from the Ministry of Education, Youth and Sports of Czech Republic; and the grants Progress Q26/LF11, SVV 260377/2017 and SVV 260257/2016 from Charles University in Prague. Part of the imaging was done at the Imaging Methods Core Facility at BIOCEV, Faculty of Science, Charles University, supported by the Czech-BioImaging large RI project (LM2015062 funded by MEYS CR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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