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Illumina MiSeq reveals the influence of blueberry malvidin-3-galactoside on fecal microbial community structure and metabolizes of liver cancer mice

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164 days ago
Illumina MiSeq reveals the influence of blueberry malvidin-3-galactoside on fecal microbial community structure and metabolizes of liver cancer mice https://t.co/iTVurwxy11 https://t.co/EXss68QWT6
Illumina MiSeq reveals the influence of blueberry malvidin-3-galactoside on fecal microbial community structure and metabolizes of liver cancer mice https://t.co/Xy4raid3Sf https://t.co/OoLPLx956z
Illumina MiSeq reveals the influence of blueberry malvidin-3-galactoside on fecal microbial community structure and metabolizes of liver cancer mice https://t.co/H7wvsWaGlw
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Supplemental Information

First batch of sequencing samples

Sequencing raw data of the first 49 stool samples

DOI: 10.7287/peerj.preprints.27429v2/supp-1

Second batch of sequencing raw data

Raw data for sequencing the last 13 stool samples

DOI: 10.7287/peerj.preprints.27429v2/supp-2

Figure 1 Venn diagram analysis

(A)Number of shared and unique OTUs in LC, LM and PC groups during the first week(B)Number of shared and unique OTUs in LC, LM and PC groups during the third week(C)Number of shared and unique OTUs in LC, HM and PC groups during the first week(D)The number of shared and unique OTUs in LC, HM and PC groups during the third week

DOI: 10.7287/peerj.preprints.27429v2/supp-3

Figure 2 Comparative analysis of family-level colonies in LM group

(A)Comparisons of LM with LC and PC in the first week(B)Comparisons of LM with LC and PC in the third week 3

DOI: 10.7287/peerj.preprints.27429v2/supp-4

Figure 3 Comparative analysis of family-level colonies in HM group

(A)Comparisons of HM with LC and PC in the first week(B)Comparisons of HM with LC and PC in the third week 3

DOI: 10.7287/peerj.preprints.27429v2/supp-5

Figure 4 Comparative analysis of genus-level colonies in LM group

(A)Comparisons of LM with LC and PC in the first week(B)Comparisons of LM with LC and PC in the third week

DOI: 10.7287/peerj.preprints.27429v2/supp-6

Figure 5 Comparative analysis of genus-level colonies in HM group

(A)Comparisons of HM with LC and PC in the first week(B)Comparisons of HM with LC and PC in the third week

DOI: 10.7287/peerj.preprints.27429v2/supp-7

Figure 6 LDA analysis

(A)Comparison of LC, LM and PC(B)Comparison of LC and HM

DOI: 10.7287/peerj.preprints.27429v2/supp-8

Figure 7 Hot-map analysis

(A)Colony Abundance Clustering of LC, LM and PC at the Third Week(B)Colony Abundance Clustering of LC, HM and PC at the Third Week

DOI: 10.7287/peerj.preprints.27429v2/supp-9

Figure 8 Comparative analysis of functional metabolism of gut microbiome

(A)Microbial metabolism in LC group during the third and first week(B)Microbial metabolism in LM group during the third and first week(C)Microbial metabolism in HM group during the third and first week(D)Microbial metabolism in PC group during the third and first week

DOI: 10.7287/peerj.preprints.27429v2/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Zhen Cheng conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

Yuehua Wang conceived and designed the experiments.

Bin Li conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Experiments were approved by the Animal Ethics Committee. Chinese Standard Guide for Laboratory Animals (GB 14922-94, GB 14923-94 and GB / T 14925-94) and the Ethics Committee of Shenyang Agricultural University (license number SYXK <Liao> 2011-0001).

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary files 1 and 2. The raw data shows the first 43 stool samples, the latter 19 stool samples in the second. In this article, all the figures showing the comparison of colonies are drawn on the computer according to the original data. The submission number SUB4908856.

Funding

This work was supported by the National Natural Science Foundation of China (31671836), the Innovative Talent Support Program for Institution of Higher Learning of Liaoning Province (LR2017038) and the Young and Middle-aged Technological Innovation Support Program of Shenyang Science and Technology Bureau (RC170247). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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