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Odoni DI, Tamayo-Ramos JA, Sloothaak J, van Heck RGA, Martins dos Santos VAP, de Graaff LH, Suarez-Diez M, Schaap PJ.2017. Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation. PeerJ Preprints5:e2742v1https://doi.org/10.7287/peerj.preprints.2742v1
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
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R. delemar ATCC20344 RNA seq assembly fasta file
Fasta file of all de novo assembled transcripts obtained from the R. delemar ATCC 20344 transcriptomics experiment.