Up-regulation of Grb2-associated binder 1 promotes hepatocyte growth factor induced endothelial progenitor cell proliferation and migration
- Published
- Accepted
- Subject Areas
- Cell Biology, Molecular Biology
- Keywords
- EPCs, Proliferation, Migration, Gab1, MAPK, HGF
- Copyright
- © 2018 Fan et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Up-regulation of Grb2-associated binder 1 promotes hepatocyte growth factor induced endothelial progenitor cell proliferation and migration. PeerJ Preprints 6:e27417v1 https://doi.org/10.7287/peerj.preprints.27417v1
Abstract
Objectives. Grb2-associated binder 1 (Gab1), a scaffolding adaptor protein, plays an important role in transmitting key signals that control cell growth, migration, and function from multiple tyrosine kinase receptors. This study was designed to investigate the influence of upregulation of Gab1 in endothelial progenitor cells (EPCs) stimulated with hepatocyte growth factor (HGF), and the underlying molecular mechanisms.
Materials and Methods. EPCs isolated from human umbilical cord blood were identified and divided into four groups. EPCs in the Control group were cultured normally; those in the Control+HGF group were treated with HGF stimulation; those in the AD-Gab1 group were transfected with adenovirus containing the Gab1 gene but not treated with HGF stimulation; and, those in the AD-Gab1+HGF group were treated with both HGF stimulation and transfection with adenovirus containing the Gab1 gene. Subsequently, Gab1 expression and proliferation and migration ability were compared for EPCs grown under different conditions. Furthermore, we measured phosphorylation levels of three key proteins Gab1, SHP2 and ERK1/2.
Results. The AD-Gab1+HGF group had the highest expression of Gab1 and higher proliferation and migration than the other three groups.
Conclusions. Upregulation of Gab1 promoted HGF-induced EPC proliferation and migration. Mechanistically, HGF stimulated Gab1 tyrosine phosphorylation in EPCs, thus leading to activation of extracellular regulated MAP kinase 1/2, which is involved in proliferation and migration signaling.
Author Comment
This is a submission to PeerJ for review.