Detailed information for all analysed individuals
Table S1. All analysed individuals with BOLD process ID, sample ID, Barcode Index numbers (BIN) and their COI haplotype membership, primers pairs used for amplification.
Divergence time for key nodes in the time-calibrated reconstruction of phylogeny
Table S2. Divergence time (Ma) for key nodes and rates of COI evolution in substitutions per site, per My estimated using Bayesian inference for different calibration schemes. Node affiliation given in Fig. S1. Calibration schemes based on secondary calibration points, standard rate and geological points (see material and methods). Lower and upper 95% highest posterior densities (95%HPD) are provided.
Results of the Bayesian node reconstruction
Table S3. Node ages (Ma) with lower and upper 95% highest posterior densities, geographical coordinates of the ancestral locations expressed as mean and standard deviation (SD), probabilities associated with occurrence in riverine (P(R)) or in lacustrine (P(L)) habitat (see Figure S1 for node identification).
K2p distances between MOTUs
Table S4. Mean Kimura two parameters (K2p) distances (below the diagonal) and standard error (SE) (above the diagonal) between MOTUs for 74 COI haplotypes. N and k = number of individual sampled and haplotypes per MOTU, respectively.
Fit of the diversification models to the Bayesian reconstruction of phylogeny
Table S5. Fit of the diversification models to Bayesian reconstruction of phylogeny, based on the Akaike information criterion (AIC).
Molecular genetic diversity and historical demography within MOTUs
Table S6. Molecular genetic diversity and historical demography based on mtDNA COI gene region (530 bp) within the 13 MOTUs (A-M). Analysis was done either at the scale of the entire MOTU (in bold) or a site or set of sites (in italic) within a MOTU.
Genetic differentiation between sites
Table S7. Genetic differentiation based on mtDNA COI gene region (530 bp) between sites (S1 vs S2) within MOTUs (A, C, E, G and K) present in more than one site within the morphospecies Gammarus roeselii in the Balkans.