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Evaluation of a toxoid fusion protein vaccine produced in plants to protect poultry against necrotic enteritis

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16 days ago
Evaluation of a toxoid fusion protein vaccine produced in plants to protect poultry against necrotic enteritis https://t.co/rpDSNjaGBq
peerJ: Evaluation of a toxoid fusion protein vaccine produced in plants to protect poultry against necrotic enteritis https://t.co/vWtmm18yKh
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Supplemental Information

DNA nucleotides sequences for PlcC-NetB fusion protein genes

DNA sequences and encoded amino acid sequences for the 6His-PlcC-NetB fusion protein either with or without ER-targeting signal peptide.

DOI: 10.7287/peerj.preprints.27390v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Joseph G Hunter conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Shyra Wilde performed the experiments, analyzed the data, prepared figures and/or tables.

Amanda M Tafoya performed the experiments.

Jamie Horsman performed the experiments.

Miranda Yousif performed the experiments.

Andrew G Diamos conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper.

Kenneth L Roland conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Hugh S Mason conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Arizona State University Institutional Animal Care and Use Committee approved this research.

Patent Disclosures

The following patent dependencies were disclosed by the authors:

PCT/US18/40632, VACCINE FOR PREVENTION OF NECROTIC ENTERITIS IN POULTRY

US 13/060,414, A DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The fusion protein nucleotide sequences are in GenBank accession numbers MH883808 and MH883809.

Data Deposition

The following information was supplied regarding data availability:

Original Western blot images for Fig. 2 and Fig. 3 are provided.

Funding

This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2016-67016-24947 from the USDA National Institute of Food and Agriculture and startup funds from ASU to Ken Roland, and by support from the Biodesign Institute at ASU for Hugh Mason. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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