Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
- Published
- Accepted
- Subject Areas
- Microbiology, Molecular Biology, Plant Science
- Keywords
- Target gene, miRNA169, Fusarium wilt, qRT-PCR, psRNATarget, target prediction
- Copyright
- © 2018 Song et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars. PeerJ Preprints 6:e27369v1 https://doi.org/10.7287/peerj.preprints.27369v1
Abstract
MicroRNAs (miRNAs) play an important role in plant resistance to pathogen infections. However, little is known about the role of miRNAs in banana Fusarium wilt, which is the most economically devastating disease in banana production. In the present study, we identified and characterized a total of 18 miR169 family members in banana (Musa acuminata L.) based on small RNA sequencing. The banana miR169 family clustered into 2 groups based on miRNA evolutionary analysis. Multiple sequence alignment indicated a high degree of sequence conservation in miRNA169 family members across 28 plant species. Computational target prediction algorithms were used to identify 25 targets of miR169 family members in banana. These targets were enriched in various metabolic pathways that include the following molecules: glycine, serine, threonine, pentose, glycerolipids, nucleotide sugars, starch, and sucrose. Through miRNA transcriptomic analysis, we found that ma-miR169a and ma-miR169b displayed high expression levels, whereas the other 16 ma-miR169 members exhibited low expression in the HG and BX banana cultivars. Further experiments indicate that there were negative relationships between ma-miR169a, ma-miR169b and their targets basing on their expression levels to Foc4(Fusarium oxysporum f. sp. cubense tropical race 4) infection in resistant cultivars. but they were low expressed in susceptive cultivars. These results suggested that the expression levels of ma-miR169a and ma-miR169b were consistent with the resistance degree of the banana cultivars to Foc4. The analysis presented here constitutes a starting point to understand ma-miR169-mediated Fusarium wilt resistance at the transcriptional level in banana and predicts possible candidate targets for the genetic improvement of banana resistance to Foc4.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Additional Files1,3,4,6,7,8
Additional file 1: MiR169 family mature sequences of all crops
Additional file 3: The targets of ma-miR169 family
Additional file 4: KEGG Pathway of the targets
Additional file 6: The FPKM values expressed of HG and BX cultivars(Reads per Kilo bases per Million reads values)
Additional file 7: The primer pairs for miRNAs and targets comfirmation and quantitative detection
Additional file 8: Expression profiles of mature ma-miR169a, ma-miR169b and their targets in root in response to Fusarium wilt
The sequences of the miR169 family members
Evolutionary analysis clustered the miR169 family.
The pathways of target genes
The pathways of target genes. 1)Glycine, serine and threonine metabolism, 2)Pentose and glucuronate interconversions, 3)Glycerolipid metabolism, 4)Amino sugar and nucleotide sugar metabolism, 5)Starch and sucrose metabolism