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Normal spermatogenesis in Fank1 (fibronectin type 3 and ankyrin repeat domains 1) mutant mice

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242 days ago
Normal spermatogenesis in Fank1 (fibronectin type 3 and ankyrin repeat domains 1) mutant mice https://t.co/yLLg2KukBH
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Supplemental Information

Table S1

SNPs associated with expression of the mRNAs of Fank1

DOI: 10.7287/peerj.preprints.27356v1/supp-1

Table S2

List of Antibodies

DOI: 10.7287/peerj.preprints.27356v1/supp-2

Supplemental File.1

Rawdata of Figure.2B

DOI: 10.7287/peerj.preprints.27356v1/supp-5

Supplemental File.2

Rawdata of Figure.2D

DOI: 10.7287/peerj.preprints.27356v1/supp-6

Supplemental File.3

Rawdata of Figure.5C, D and E

DOI: 10.7287/peerj.preprints.27356v1/supp-7

Supplemental File.4

Rawdata of Figure.5F

DOI: 10.7287/peerj.preprints.27356v1/supp-8

Supplemental File.5

Rawdata of Figure.6

DOI: 10.7287/peerj.preprints.27356v1/supp-9

Supplemental File.6

Rawdata of Figure.7

DOI: 10.7287/peerj.preprints.27356v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jintao Zhang performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Xin Zhang performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables.

Yue Zhang performed the experiments, analyzed the data.

Wentao Zeng performed the experiments.

Shuqin Zhao performed the experiments.

Mingxi Liu conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Institutional Animal Care and Use Committee of Nanjing Medical University (China)

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary files. The raw data shows no significant changes in epididymal sperm content and the number of apoptotic cells were observed in Fank1-/- homozygotes. In addition, a different pattern of Dusp1, Klk1b21 and Klk1b27 mRNA expression was detected in Fank1-knockout testis.

Funding

This work was funded by the Natural Science Foundation of China (31571536 and 31771654), The Natural Science Foundation of the Jiangsu Higher Education Institutions of China (16KJA310003), Natural Science Foundation of Jiangsu Province (No. BK20150990) and Qing Lan Project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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