Voucher specimen information
Data regarding voucher specimen collection dates and localities and repositories.
Data file used in final phylogeny and divergence time estimation analyses
Nexus file of alined sequences used for analyses.
Table S1. Taxonomic and Genetic Sampling
Taxonomic and Genetic Sampling. Exemplars used from Deans et al. (2006) are listed with the reference from that paper (Deans ID) beside the internal voucher number (Extract ID). Included genes for each taxon are marked with an x. Gene codes: 28S for 28S rDNA; AM2 for alpha-mannosidase II; CAD1 and CAD2 for carbamoyl-phosphate sythetase-asparate transcarbamoylase-dihydroorotase (CAD) (for amplicon regions for each segment, see Figure 1); RPS23 for Ribosomal Protein S23; COI for cytochrome oxidase I; and 16S for 16S rDNA
Primers and PCR protocols used in this study
For protein-coding genes, the Primer Name is based off the amino acid position in the Apis mellifera CDS for that gene. An Alternate Primer Name is given if a different name was used in a previously published study or in-house.
Average nucleotide composition (%) across genes
Highlighted genes indicate that the test for base composition homogeneity across taxa was falsified. Statistics are provided below the table.
Recovered clades across individual genes and concatenated analyses (All genes)
Monophyletic clades are marked with an x, which is shaded grey if the posterior probability was 0.95 or greater. N/A indicates that there was a missing taxon so the clade could not be recovered. A “C” preceding a taxon name in a cell indicates that a congruent clade was recovered even though the indicated taxon was missing. Taxon Abbreviations are as follows: Mic (Micrevania); Bra (Brachygaster); Pro (Prosevania); Tri (Trissevania); Eva (Evania); Zeu (Zeuxevania); Par (Parevania); CEv (Evaniscus); Dec (Decevania); Sem (Semaeomyia); Hyp (Hyptia); Alo (Alobevania); LEv (Evaniella); Sze (Szepligetella); Aca (Acanthinevania).
Individual gene trees
Bayesian analysis of each gene individually for Evaniidae. Posterior probabilities are listed beside each node
Figures that illustrate the unique sequence motifs (RPS23 and 28S rDNA) for certain lineages of Evaniidae
Estimated divergence times for Evaniidae under a normal distribution
Posterior probabilities are listed beside the relevant node. The 95% HDP for all clades is listed in Table 2. The scale is in millions of years.
Figure Maximum Likelihood results
Maximum Likelihood analyses of the concatenated dataset. Bootstrap values are listed beside each node.