Complete chloroplast genome sequence and structural analysis of the medicinal plant Lycium chinense Mill

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1 Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
2 Pharmaceugtical school, YouJiang Medical University For Nationalities, Baise, Guangxi, China
DOI
10.7287/peerj.preprints.27305v1
Published
Accepted
Subject Areas
Genomics, Molecular Biology, Plant Science
Keywords
Lycium chinense Mill, chloroplast genome, phylogenetic analysis
Copyright
© 2018 Yang et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Yang Z, Huang Y, Zheng X, Huang S, Liang L. 2018. Complete chloroplast genome sequence and structural analysis of the medicinal plant Lycium chinense Mill. PeerJ Preprints 6:e27305v1

Abstract

Lycium chinense Mill, an important Chinese herbal medicine, is emphasized as a healthy food and is widely used as a dietary supplement. Here we sequenced and analyzed the complete chloroplast (CP) genome of the L. chinense, which is 155,756 bp in length and with 37.8% GC content. This CP genome consists of a pair of inverted repeat regions (IRa and IRb) of 25,476 bp, separated by a large single-copy region (LSC) and a small single-copy region (SSC), with length of 86,595 and 18,209 bp, respectively. Annotation results revealed that the L. chinense CP genome contains 114 genes, 16 of which are duplicated genes. Most of the 85 protein-coding genes have a usual ATG start codon, except for 3 genes including rps12, psbL and ndhD. Furthermore, most of the simple sequence repeats (SSRs) are short polyadenine or polythymine repeats that contribute to the high AT content of the chloroplast genome. Revealing of the complete sequences and annotation of the L. chinense chloroplast genome will facilitate phylogenic, population and genetic engineering research investigations involving this particular species.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Codon usage of L. chinense chloroplast genome

DOI: 10.7287/peerj.preprints.27305v1/supp-1

RNA editing predicted in the chloroplast genomes of L. chinense by the PREP program

DOI: 10.7287/peerj.preprints.27305v1/supp-2

Chloroplast genomes used to study their phylogeny relationship in this study

DOI: 10.7287/peerj.preprints.27305v1/supp-3