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Divergent patterns of ocular development and gene expression in the evolution of a subterranean salamander

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238 days ago
Divergent patterns of ocular development and gene expression in the evolution of a subterranean salamander https://t.co/k5B55tr2Jl
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Supplemental Information

E. rathbuni Pax6 and Shh expression at stage 37 and 40

Dorsal view of eye development in E. rathbuni. Pax6 and Shh expression at stage 37 (A&B), and 40 (C&D).

DOI: 10.7287/peerj.preprints.27301v1/supp-1

E. rathbuni Pax6 and Shh expression at stage 37 and 40

Dorsal view of eye development in E. sosorum. Pax6 and Shh expression at stage 37 (A&B), and 40 (C&D).

DOI: 10.7287/peerj.preprints.27301v1/supp-2

Eye measurements

Each data point represents the average of the right and left eye standardized by head length for each species and respective stage, early development and adult.

DOI: 10.7287/peerj.preprints.27301v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ruben U. Tovar conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Valentin Cantu analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Brian P. Fremaux analyzed the data, prepared figures and/or tables.

Pedro Gonzalez analyzed the data.

Dana M. García conceived and designed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Institutional Animal Care and Use Committee

Data Deposition

The following information was supplied regarding data availability:

The raw data images are provided in the supplementary files 1-2, and raw measurements in supplementary file 3.

Funding

This work was supported by the East Texas Herpetological Society, South Texas Herpetological Society, Southwestern Association of Naturalist Howard McCarley Student Research Award, Herpetologists’ League Jones-Lovich in Southwestern Herpetology Award, and Texas State University Thesis Research Support Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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