Identification and expression analysis of chemosensory genes in the citrus fruit fly Bactrocera (Tetradacus) minax
- Published
- Accepted
- Subject Areas
- Agricultural Science, Bioinformatics, Entomology, Genomics
- Keywords
- Bactrocera (Tetradacus) minax, Odorant-binding protein, Chemosensory proteins, Odorant receptors, Bactrocera dorsalis
- Copyright
- © 2018 Cheng et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Identification and expression analysis of chemosensory genes in the citrus fruit fly Bactrocera (Tetradacus) minax. PeerJ Preprints 6:e27297v1 https://doi.org/10.7287/peerj.preprints.27297v1
Abstract
The citrus fruit fly Bactrocera (Tetradacus) minax is a major and devastating agricultural pest in Asian subtropical countries. Previous studies have shown that B. minax interacts with hosts via an efficient chemosensory system. However, knowledge regarding the molecular components of the B. minax chemosensory system has not yet been well established. Herein, based on our newly generated whole-genome dataset for B. minax and by comparison with the characterized genomes of 6 other fruit fly species, we identified, for the first time, a total of 25 putative odorant-binding receptors (OBPs), 4 single-copy chemosensory proteins (CSPs) and 53 candidate odorant receptors (ORs). To further survey the expression of these candidate genes, the transcriptomes from three developmental stages (larvae, pupae and adults) of B. minax and Bactrocera dorsalis were analyzed. We found that 1) at the adult developmental stage, there were 14 highly expressed OBPs (FPKM>100) in B. dorsalis and 7 highly expressed OBPs in B. minax; 2) the expression of CSP3 and CSP4 in adult B. dorsalis was higher than that in B. minax; and 3) most of the OR genes exhibited low expression at the three developmental stages in both species. This study on the identification of the chemosensory system of B. minax not only enriches the existing research on insect olfactory receptors but also provides new targets for preventative control and ecological regulation of B. minax in the future.
Author Comment
This is a submission to PeerJ for review.