Description and classification of bivalve mollusks hemocytes: a computational approach

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1 National Scientific Center of Marine Biology FEB RAS, Vladivostok, Russia
2 Far Eastern Federal University, Vladivostok, Russia
DOI
10.7287/peerj.preprints.27258v1
Published
Accepted
Subject Areas
Bioinformatics, Cell Biology, Computational Biology, Marine Biology
Keywords
hemocytes, morphometry, bivalve, fractal analysis, cell morphology
Copyright
© 2018 Karetin et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Karetin YA, Kalitnik AA, Safonova AE, Cicinskas E. 2018. Description and classification of bivalve mollusks hemocytes: a computational approach. PeerJ Preprints 6:e27258v1

Abstract

The fractal formalism in combination with linear image analysis enables statistically significant description and classification of “irregular” (in terms of Euclidean geometry) shapes, such as, outlines of in vitro flattened cells. We developed an optimal model for classifying bivalve Spisula sachalinensis and Callista brevisiphonata immune cells, based on evaluating their linear and non-linear morphological features: dimensional characteristics (area, perimeter), various parameters of cell bounding circle, convex hull, cell symmetry, roundness, and a number of fractal dimensions and lacunarities evaluating the spatial complexity of cells. Proposed classification model is based on Ward’s clustering method, loaded with highest multimodality index factors. This classification scheme groups cells into three morphological types, which can be distinguished both visually and by several linear and quasi-fractal parameters.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Lists of parameters, their explanation and computation

Full lists of parameters used in this study, their explanation and computation

DOI: 10.7287/peerj.preprints.27258v1/supp-1