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Supplemental Information

Figure S1 The sampling site of P. tentoria in northeast India. Map not to scale and manually prepared by the first author (S.K.) with the help of Google Map ( https://www.google.com/maps ) and Adobe Photoshop CS 8.0

The riverine systems are marked by blue color, national boundary between state Arunachal Pradesh and Assam is marked by Black dotted line, international boundary of India and Myanmar is marked by Red dotted line, two National Parks are marked by Green colors, sampling sites with geographical coordinates is marked by orange dot. The original template of the topographic map of India used here is copied under the following attribution: created by Yug (Own work) [CC BY-SA 3.0 http://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons; file URL: https://upload.wikimedia.org/wikipedia/commons/b/b9/Wikimaps_atlas-India-topographic_map-color-blank.jpg; page URL: https://commons.wikimedia.org/wiki/File%3AWikimaps_atlas-India-topographic_map-color-blank.jpg.

DOI: 10.7287/peerj.preprints.27202v1/supp-1

Figure S2 A pictorial overview of the methodologies used for sequencing and analysis ofP. tentoria mitogenome and bioanalyzer profiles after sonication of enriched mitochondrial DNA sample and libraries

DOI: 10.7287/peerj.preprints.27202v1/supp-2

Figure S3 Codon distribution 32 geoemydid species including P. tentoria . CDspT=codons per thousand codons

DOI: 10.7287/peerj.preprints.27202v1/supp-3

Figure S4 Putative secondary structures for 22 tRNA genes in mitochondrial genome of P. tentoria

DOI: 10.7287/peerj.preprints.27202v1/supp-4

Table S1: List of complete mitochondrial sequences acquired from GenBank database used in the current comparative analysis

DOI: 10.7287/peerj.preprints.27202v1/supp-5

Table S2: Start and stop codons of PCGs in 32 geoemydid species

DOI: 10.7287/peerj.preprints.27202v1/supp-6

Table S3: Comparison of codon usage within the mitochondrial genome of 32 geoemydid turtles

DOI: 10.7287/peerj.preprints.27202v1/supp-7

Table S4: Codon distribution in 32 geoemydid species. CDspT = codons per thousand codons

DOI: 10.7287/peerj.preprints.27202v1/supp-8

Table S5: Comparision of overlapping and intergenic spacer regions of the studied geoemydid turtles

DOI: 10.7287/peerj.preprints.27202v1/supp-9

Table S6: Anticodons of tRNA genes in 32 geoemydid species

DOI: 10.7287/peerj.preprints.27202v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Shantanu Kundu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Vikas Kumar conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Kaomud Tyagi performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Rajasree Chakraborty performed the experiments, prepared figures and/or tables.

Iftikar Rahaman analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Kailash Chandra contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Arunachal Pradesh Biodiversity Board and Zoological Survey of India, Kolkata

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The genomic DNA of Pangshura tentoria were deposited in Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, Kolkata under IDs ‘ZSI_NFGR-TT7’. The following information was supplied regarding the availability of DNA sequences: The complete mitogenome of Pangshura tentoria is deposited in GenBank of NCBI under accession number MH795989.

Data Deposition

The following information was supplied regarding data availability:

The following information was supplied regarding the availability of DNA sequences: The complete mitogenome of Pangshura tentoria is deposited in GenBank of NCBI under accession number MH795989.

Funding

This work was supported by the Ministry of Environment, Forest and Climate Change (MoEF&CC), Zoological Survey of India (ZSI) in-house project, ‘National Faunal Genome Resources (NFGR)’. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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