Visitors   Views   Downloads

Comparative analysis and characterization of the gut microbiota of four farmed snakes from southern China

View preprint
95 days ago
PJpreprint: Comparative analysis and characterization of the gut microbiota of four farmed snakes from southern China https://t.co/6yx2kNhWao
95 days ago
Comparative analysis and characterization of the gut microbiota of four farmed snakes from southern China https://t.co/Ti4Rt89Oz1
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

Supplemental Information

Operational taxonomic units (OTUs) rank curve

Na: Naja atra group, Pmu: Ptyas mucosus group, Ec: Elaphe carinata group, and Da: Deinagkistrodon acutus group.

DOI: 10.7287/peerj.preprints.27193v1/supp-1

Venn diagram indicating the shared and unique operational taxonomic units (OTUs) across all species

Na: Naja atra group, Pmu: Ptyas mucosus group, Ec: Elaphe carinata group, and Da: Deinagkistrodon acutus group.

DOI: 10.7287/peerj.preprints.27193v1/supp-2

Relative abundance of bacterial classes across four snake species

Na: Naja atra group, Pmu: Ptyas mucosus group, Ec: Elaphe carinata group, and Da: Deinagkistrodon acutus group.

DOI: 10.7287/peerj.preprints.27193v1/supp-3

Relative abundance of bacterial orders across four snake species

Na: Naja atra group, Pmu: Ptyas mucosus group, Ec: Elaphe carinata group, and Da: Deinagkistrodon acutus group.

DOI: 10.7287/peerj.preprints.27193v1/supp-4

Relative abundance of bacterial families across four snake species

Na: Naja atra group, Pmu: Ptyas mucosus group, Ec: Elaphe carinata group, and Da: Deinagkistrodon acutus group.

DOI: 10.7287/peerj.preprints.27193v1/supp-5

Principal component analysis of the microbiota composition across four snake species

The variation explained by the plotted principal component is indicated by the axis labels. Na: Naja atra group, Pmu: Ptyas mucosus group, Ec: Elaphe carinata group, and Da: Deinagkistrodon acutus group.

DOI: 10.7287/peerj.preprints.27193v1/supp-6

A list of bacteria at genus level

DOI: 10.7287/peerj.preprints.27193v1/supp-8

The description of alpha diversity

DOI: 10.7287/peerj.preprints.27193v1/supp-9

Additional Information

Competing Interests

The authors declare that they have no competing interests. Xinguo Gong is chairman of Qiyang Gong Xinguo breeding Co., Ltd.

Author Contributions

Bing Zhang conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, bing Zhang wrote the paper..

Jing Ren performed the experiments, analyzed the data, jing Ren wrote the paper..

Daode Yang conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Shuoran Liu conceived and designed the experiments, authored or reviewed drafts of the paper.

Xinguo Gong performed the experiments.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

This study was performed in accordance with the recommendations of the Institution of Animal Care and the Ethics Committee of Central South University of Forestry and Technology (approval number: CSUFT NS # 20175167) .

Data Deposition

The following information was supplied regarding data availability:

The sample detail (Sample name, Tag number and OTU number), a list of bacteria at genus level and the description of alpha diversity are provided in the supplementary files(Table S1, Table S2 and Table S3, respectively). The OTU rank curve indicating that bacterial abundance and evenness differed among samples are provided in the Figure S1. The shared and unique operational taxonomic units (OTUs) across all species are provided in the Figure S2. Relative abundance of bacterial at classes, orders and families level across four snake species are provided in the Figure S3-5. Principal component analysis of the microbiota composition across four snake species are provided in the Figure S6.

Funding

This study was funded by the National Natural Science Foundation of China (No.31472021) and the Project for Wildlife Conservation and Management of the State Forestry Administration of China (No.16180617). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies