Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens

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1 Genetic Department, Faculty of Agriculture., Ain Shams University, Cairo, Egypt
2 Institute of Parasitology, Biology Centre, Czech academy of Science, České Budějovice, Czech Republic
3 Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR) of Italy, Palermo, Italy
4 Central Lab. of Organic Agriculture, Agricultural Research Center, Giza, Egypt
5 Botany and Microbiology Dept., Faculty of Science, Helwan University, Cairo, Egypt
6 Microbiology Dept., Faculty of Agriculture, Ain Shams University, Cairo, Egypt
DOI
10.7287/peerj.preprints.27186v1
Published
Accepted
Subject Areas
Genomics, Microbiology, Virology
Keywords
Bacteriophage, Biological Stability, Whole Genome Sequence, Comparative Genome, NGS
Copyright
© 2018 Sharaf et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Sharaf A, Mercati F, Elmaghraby I, Elbaz R, Marei E. 2018. Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens. PeerJ Preprints 6:e27186v1

Abstract

Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens We report biological and physiochemical features and use NGS to provide the complete annotated genomes for two new strains (Sf1 and Sf3) of the virulent phage Streptomyces flavovirens, isolated from Egyptian soil samples. The S. flavovirens phages (Sf1 and Sf3) show high adsorption rates (82 and 85 %, respectively), indicating a strong specificity to their host, and their burst sizes were 1.95 and 2.49 virions per mL. The phage genomes are parts of a singleton cluster with sizes of 43,150 bp and 60,934 bp, respectively. the assignment of possible functions to 19 and 28 putative ORFs were identified, which included phage structural proteins, lysis components and metabolic proteins. Comparative genomic analysis revealed significant homology between the two phages and the closest Streptomyces phage (VWB phages). However, the phylogenetic analysis highlighted that the isolated phages belong to the BG Streptomyces phage group but are clearly separated, representing a novel sub-cluster.

Author Comment

This poster is a part of a conference proceedings - CzechoSlovak virology conference, České Budějovi, Feburary 2017.