Characteristics of the complete mitochondrial genome of Suhpalacsa longialata (Neuroptera, Ascalaphidae) and its phylogenetic implications

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1 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, China
2 Key lab of wildlife biotechnology, conservation and utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, Zhejiang, China
3 Department of Biology, Carleton University, Ottawa, Ontario, Canada
DOI
10.7287/peerj.preprints.27183v2
Published
Accepted
Subject Areas
Entomology, Genomics, Molecular Biology
Keywords
Neuroptera, Ascalaphidae, Mitochondrial genome, Phylogenetic relationship
Copyright
© 2018 Gao et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Gao X, Cai Y, Yu D, Storey KB, Zhang J. 2018. Characteristics of the complete mitochondrial genome of Suhpalacsa longialata (Neuroptera, Ascalaphidae) and its phylogenetic implications. PeerJ Preprints 6:e27183v2

Abstract

The owlflies (Family Ascalaphidae) belong to the Neuroptera but are often mistaken as dragonflies because of morphological characters. To date, only three mitochondrial genomes of Ascalaphidae, namely Libelloides macaronius; Ascaloptynx appendiculatus; Ascalohybris subjacens, are published in GenBank, meaning that they are greatly under-represented in comparison with the 430 described species reported in this family. In this study, we sequenced and described the complete mitochondrial genome of Suhpalacsalongialata (Neuroptera, Ascalaphidae). The total length of the S.longialata mitogenome was 15,911 bp, which is the longest known to date among the available family members of Ascalaphidae. However, the size of each gene was similar to the other three Ascalaphidae species. The S. longialata mitogenome included a transposition of tRNACys and tRNATrp genes and formed an unusual gene arrangement tRNACys-tRNATrp-tRNATyr(CWY). It is likely that the transposition occurred by a duplication of both genes followed by random loss of partial duplicated genes. The nucleotide composition of the S.longialata mitogenome was as follows: A=41.0%, T=33.8%, C=15.5%, G=9.7%. Both BI and ML analyse strongly supported S. longialata as a sister clade to (Ascalohybris subjacens + L. macaronius), and indicated that Ascalaphidae is not monophyletic.

Author Comment

We revised the manuscript according to the reviewers' comments and suggestion.

Supplemental Information

Table S1. Universal and specific primers used to amplify the mitochondrial genome of S.longialata

All universal primers were modified according to Simon et al. (2006), Zhang et al. (2008) and Zhang et al. (2018) by comparing to known mayfly mitochondrial genomes. The orientation of primers is as shown in Fig. 1.

DOI: 10.7287/peerj.preprints.27183v2/supp-1

Table S2. Species used to construct the phylogenetic relationships along with GenBank accession numbers

DOI: 10.7287/peerj.preprints.27183v2/supp-2

Table S3. The codon number and relative synonymous codon usage (RSCU) in S.longialata mitochondrial protein-coding genes

DOI: 10.7287/peerj.preprints.27183v2/supp-3

The unpublished sequence for review is attached as a Supplemental File, which GenBank number is MH361300

DOI: 10.7287/peerj.preprints.27183v2/supp-4