Visitors   Views   Downloads

Carcinogen treatment leads to mitotic defects and arrest in cancer and noncancer cells

View preprint
404 days ago
Carcinogen treatment leads to mitotic defects and arrest in cancer and noncancer cells https://t.co/OdPryNrKgf
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

Supplemental Information

A549 cell dividing normally, no discernable mitotic defect

A549 cell treated with 20 μM vinyl chloride for 24 hours before being labeled with SiR-DNA. 21 stacks in the Z plane were imaged every three minutes for a total duration of 1 hour, 24 minutes.

DOI: 10.7287/peerj.preprints.27177v1/supp-1

A549 cell with prometaphase/metaphase laging chromosome fails to complete mitosis

A549 cell treated with 20 μM vinyl chloride for 24 hours before being labeled with SiR-DNA. 21 stacks in the Z plane were imaged every three minutes for a total duration of 2 hours.

DOI: 10.7287/peerj.preprints.27177v1/supp-2

A549 cell with anaphase bridge that completes mitosis

A549 cell treated with 20 μM vinyl chloride for 24 hours before being labeled with SiR-DNA. 23 stacks in the Z plane were imaged every three minutes for a total duration of 4 hours 40 minutes.

DOI: 10.7287/peerj.preprints.27177v1/supp-3

UPCI:SCC103 cell with no discernable mitotic defect, fails to complete mitosis

UPCI:SCC103 cell treated with 20 μM vinyl chloride for 24 hours before being labeled with SiR-DNA. 22 stacks in the Z plane were imaged every three minutes for a total duration of 1 hour 54 minutes. The cell appears to eject chromosomal material after failing to proceed to anaphase.

DOI: 10.7287/peerj.preprints.27177v1/supp-4

UPCI:SCC103 cell with prometaphase lagging chromosome, fails to complete mitosis

UPCI:SCC103 cell treated with 20 μM vinyl chloride for 24 hours before being labeled with SiR-DNA. 24 stacks in the Z plane were imaged every three minutes for a total duration of 2 hours 36 minutes.

DOI: 10.7287/peerj.preprints.27177v1/supp-5

UPCI:SC103 cell with anaphase bridge that completes mitosis

UPCI:SCC103 cell treated with 20 μM vinyl chloride for 24 hours before being labeled with SiR-DNA. 21 stacks in the Z plane were imaged every three minutes for a total duration of 1 hour, 18 minutes.

DOI: 10.7287/peerj.preprints.27177v1/supp-6

Viability counts after treatment with Fulvestrant or vinyl chloride

Raw data for viability counts for A549, UPCI:SCC103, and GM03349 cells after treatment with increasing concentrations of Fulvestrant or Vinyl Chloride for 24, 36, or 48 hours. Viability was measured using flow cytometry. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-7

Mitotic defect frequency after Fulvestrant treatment

Raw data: A549, UPCI:SCC103 and GM03349 cells were treated with increasing concentrations of Fulvestrant for 24, 36, or 48 hours and scored for mitotic index. Cell counts and percentages are recorded. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-8

Mitotic Index after vinyl chloride treatment

Raw data: A549, UPCI:SCC103 and GM03349 cells were treated with increasing concentrations of vinyl chloride for 24, 36, or 48 hours and scored for mitotic index. Cell counts and percentages are recorded. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-9

Frequency of mitotic defects after Fulvestrant treatment

Raw data: A549, UPCI:SCC103 and GM03349 cells were treated with increasing concentrations of Fulvestrant for 24, 36, or 48 hours and scored for frequency of mitotic defects. Cell counts and percentages are recorded. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-10

Frequency of mitotic defects after vinyl chloride treatment

Raw data: A549, UPCI:SCC103 and GM03349 cells were treated with increasing concentrations of vinyl chloride for 24, 36, or 48 hours and scored for frequency of mitotic defects. Cell counts and percentages are recorded. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-11

Live Cell Imaging Outcomes

Raw Data: Summary of all movies generated. A549, UPCI:SCC103, and GM03349 cells were imaged (either with or without 24 hours exposure to 20 μM vinyl chloride) and outcomes recorded for both presence of mitotic defects and completion of mitosis. Movies were analyzed frame-by-frame for scoring purposes.

DOI: 10.7287/peerj.preprints.27177v1/supp-12

Mitotic Index after recovery from vinyl chloride treatment

Raw data: A549, UPCI:SCC103 and GM03349 cells were treated with 20 μM vinyl chloride for 24 hours before washout with fresh media. Cells were fixed at 0, 24, 48, 72, and 96 hours post-washout and scored for mitotic index. Cell counts and percentages are recorded. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-13

Frequency of mitotic defects after recovery from vinyl chloride treatment

Raw data: A549, UPCI:SCC103 and GM03349 cells were treated with 20 μM vinyl chloride for 24 hours before washout with fresh media. Cells were fixed at 0, 24, 48, 72, and 96 hours post-washout and scored for frequency of mitotic defects. Cell counts and percentages are recorded. T-tests were performed comparing each experimental condition to control values individually, p-values are noted.

DOI: 10.7287/peerj.preprints.27177v1/supp-14

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Rebecca G Hartling conceived and designed the experiments, performed the experiments, analyzed the data, approved the final draft.

Christian J Pacheco conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Emily A Bystrak conceived and designed the experiments, performed the experiments, approved the final draft.

Nicholas J Quintyne conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Raw data for mitotic indices, mitotic defect frequencies and movie outcomes are uploaded as Excel files.

Funding

RGH and EAB were partially supported by summer research fellowships from the Holmberg Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies