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Supplemental Information

General characteristics of the lakes

% – contribution in the littoral zone (Sm – Sphagnum mat, Di – Sparse macrophytes, De - Dense macrophytes, A – sandy bottom), T – Stage of succession (O – oligohumic, M – mesohumic, P – polyhumic), S – number of species.

DOI: 10.7287/peerj.preprints.27167v1/supp-1

Quantitative occurrence of beetles in humic lake

N – number of individuals, NS – number of samples. L – number of lakes, Bs – body size class, F – functional group (P – predators, S – saprophages, F – polyphages), P – Legal protection, EN,VU, LC – threat status (Polish Red List), min – minimum abundance of the species in a single sample (excluded samples where the species did not occur), max – abundance of the species in a single sample, Mean – average abundance of the species in a single sample, SD – standard deviation.

DOI: 10.7287/peerj.preprints.27167v1/supp-2

Results of HSD Tuckey post hoc test for GLM repeated measure Anova

Influence of significant interaction between habitats and Body size on A) abundance of beetles, B) number of species. SN - Subclass number, BS - Body size class (1-very small beetles, 2-small, 3-medium, 4-large, 5-very large), Habitat: Sphagnum – compacted Sphagnum mat, dense – dense macrophytes zone, sparse - sparse macrophytes zone,

DOI: 10.7287/peerj.preprints.27167v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Joanna Pakulnicka conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Andrzej Zawal performed the experiments, analyzed the data, approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Field studies were conducted in accordance with field-study-approvals: Certificate of RDOS in Olsztyn (WOPN.070.21.2018.AKI), WNP certificate (PNE 08-070 / 18) and ZPK Certificate (060.1.2018).

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary files (Appendix 4). The raw data shows all water beetles, their abundance collected in 40 lakes, and body size and weight. In addition, raw data show the habitat characteristics of the taken samples (also coded). All data was used for all statistical analyzes.

The raw data are provided in Appendix 4.


The authors received no funding for this work.

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