Cryopreservation of Limnoperna fortunei (golden mussel) sperm with polyethylene glycol
- Published
- Accepted
- Subject Areas
- Aquaculture, Fisheries and Fish Science, Cell Biology
- Keywords
- sperm, cryopreservation, Limnoperna fortunei, liquid nitrogen, polyethylene glycol, thawing
- Copyright
- © 2018 Markovic et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Cryopreservation of Limnoperna fortunei (golden mussel) sperm with polyethylene glycol. PeerJ Preprints 6:e27165v2 https://doi.org/10.7287/peerj.preprints.27165v2
Abstract
Background. Insufficient quantities of freshly harvested Limnoperna fortunei gametes and embryos constrain reproduction research in the laboratory. Cryopreservation would allow the accumulation and storage of gametes when they are available. The lack of available cryopreservation protocol for Limnoperna fortunei in the literature led our research group to undertake a study to establish which cryoprotective agents can be might be most useful for cryopreservation of this species’ sperm. Methods. 2%, 5%, and 10% concentration of ethylene glycol, dimethyl sulfoxide, glycerol, and polyethylene glycol 4000 as well as 0.2 M glucose, sucrose, and trehalose (mixed into the 10% concentration of the aforementioned agents) were tested in a 1:1 ratio with sperm, in 0.25 ml straws, frozen in liquid nitrogen. Results. After 48 hours the best survival rate was in the samples with 10% polyethylene glycol 4000, 36.1%, which also resulted in viable sperm after 7 and 15 days.
Author Comment
This is a preprint submission to PeerJ Preprints. The changes which are made between this and previous version of the preprint: the text is organised in two columns and the tables are inserted inside the text. The hyperlink with DOI was added inside the abstract box.