Open-Source bioreactor controller for bacterial protein expression
- Published
- Accepted
- Subject Areas
- Bioinformatics, Biotechnology
- Keywords
- Arduino Bioreactor, Open-Source Bioreactor, protein expression
- Copyright
- © 2018 Marinescu et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Open-Source bioreactor controller for bacterial protein expression. PeerJ Preprints 6:e27150v1 https://doi.org/10.7287/peerj.preprints.27150v1
Abstract
Growing microorganisms for laboratory experiments or industrial biotechnological process is an activity which involves the use of bioreactors. Although there are many commercially available equipment, most of them lack the flexibility of an open-source solution. This work proposes a cost effective Arduino based bioreactor controller for growing suspended microbial cells. To exemplify its functionality, this study provides the parts list and schematics necessary to make a functional laboratory scale bench top stirred tank bioreactor. Using the built prototype, an E. coli culture is grown maintaining the preset parameters, protein expression is induced and culture is harvested at preset culture density. Automatically recorded process data shows stable environmental parameters and reliable bacterial growing curve.
Author Comment
This is a preprint submission to PeerJ Preprints.
Supplemental Information
Supplement 1
Example log file as recorded on micro SD card.
Supplement 2
Example of relevant sections from the serial port log.
Supplement 3
Computer Assisted Design file for 3D printing of the plastic support for the immersed version of OD600 sensor.
Supplement 4
The process specific variables setup file.
Supplement 5
Example of micro SD card file storing non volatile memory variable (e.g. reference light intensity (l0) analog value) automatically saved at process startup.
Supplement 6
The complete Arduino source code for the proposed bioreactor controller.