Host specificity determines the assemblage of root endophytic bacteria of plants growing in metal contaminated soil
- Published
- Accepted
- Subject Areas
- Biodiversity, Ecology, Microbiology
- Keywords
- Diversity, endophytic bacteria, Ageratina adenophora, Alnus nepalensis, Arthraxon hispidus, Lead-zinc mine tailings
- Copyright
- © 2018 Song et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Host specificity determines the assemblage of root endophytic bacteria of plants growing in metal contaminated soil. PeerJ Preprints 6:e27143v1 https://doi.org/10.7287/peerj.preprints.27143v1
Abstract
The diversity of endophytic bacteria colonizing the roots of an invasive plant, a pioneer plant, and an endemic plant from varied plant communities developing in a lead-zinc mine tailing pond, southwestern China, was analyzed by the culture-independent method. A total of 1650 16S rDNA sequences were screened for the establishment of four clone libraries, from the pure stands of Arthraxon hispidus (LS-Ah) and Ageratina adenophora (LS-Aa), and a mixed stand of A. adenophora (LC-Aa) and Alnus nepalensis (LC-An) (co-dominant community), respectively. Phylogenetic analysis revealed that the sequences were clustered into at least 17 phylogroups, which consisted of alpha, beta, gamma, delta subclasses of the Proteobacteria, Tenericutes, Bacteroidetes, Chloroflexi, Actinobacteria, Spirochaetes, Chlamydiae, Firmicutes, Deinococcus-Thermus, Planctomycetes, Nitrospirae, Gemmatimonadetes and unclassified bacteria Candidatus Saccharibacteria. The dominant phylum was Proteobacteria (50.49% of the total clones), and the dominant genus was Candidatus Phytoplasma (19.94% of the total clones). The invasive plant (A. adenophora) accumulated more parasitic endophytic bacteria (Phytoplasma) than the other two native plants. Phylogenetic structures of the four 16S rDNA clone libraries were distinct with their similarity indices being less than 0.5. The results also revealed that the dominant phyla and dominant genera in the four clone libraries varied a lot, and the endemic grass harbored a higher diversity of endophytic bacteria than the pioneer and invasive plants, the host-specificity took a more important role in shaping the endophytic bacteria community than the habitats in the metal stressed environment.
Author Comment
This is a submission to PeerJ for review.
