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Prevalence of Wolbachia in 10 Tenebrionidae stored product insects and infection density dynamics in confused flour beetle Tribolium confusum (Jaquelin Du Val) (Coleoptera: Tenebriondae)

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RT @WolbachiaPapers: Prevalence of Wolbachia in 10 Tenebrionidae stored product insects and infection density dynamics in confused flour be…
Prevalence of Wolbachia in 10 Tenebrionidae stored product insects and infection density dynamics in confused flour beetle Tribolium confusum (Jaquelin Du Val) (Coleoptera: Tenebriondae) https://t.co/d1wj1v2aLX
296 days ago
Prevalence of Wolbachia in 10 Tenebrionidae stored product insects and infection density dynamics in confused flour beetle Tribolium confusum (Jaquelin Du Val) (Coleoptera: Tenebriondae) https://t.co/3fjRgS3nHH
Prevalence of Wolbachia in 10 Tenebrionidae stored product insects and infection density dynamics in confused flour beetle Tribolium confusum (Jaquelin Du Val) (Coleoptera: Tenebriondae) https://t.co/KCAlnolxk5
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Supplemental Information

Table 1. Wolbachia infectionstatus in Tenebrionidae stored product pests in China

DOI: 10.7287/peerj.preprints.27059v1/supp-1

Table2. CI expression and reproduction parameters of antibiotic treated( w- ) and untreated( w+ ) Tribolium confusum

♂, male; ♀, female; w+, Wolbachia-infected beetle; w-, Wolbachia-uninfected beetle (Tetracycline treatment); Eggs number is laid by one pair beetles in 30 days. Mean ± SD followed by different letters in the same column indicate significant difference(Turkey HSD test, P<0.05).

DOI: 10.7287/peerj.preprints.27059v1/supp-2

Fig.1 Dynamicsof Wolbachia infection during thedevelopment of Tribolium confusum

Reported values are mean ± SD for wsp copies per mg insect mass (fresh weight). The error bars indicate standard deviation (SD) (n=6). Adults were females.

DOI: 10.7287/peerj.preprints.27059v1/supp-3

Fig.2 Comparison of Wolbachia infection density in Tribolium confusum male and female pupae and adults

(* indicates significant difference between two groups, NS indicates no significant difference, Independent samples t-test, P<0.05).

DOI: 10.7287/peerj.preprints.27059v1/supp-4

Fig.3Relative amounts of Wolbachia population density in head,thorax and abdomen of T. confusum.

Data expressed in terms of number of wsp copies per individual body part.

DOI: 10.7287/peerj.preprints.27059v1/supp-5

Fig.4 Comparisonof the population density of Wolbachia in different tissues of T.confusum

Data expressed in terms of number of wsp copies per ng template DNA. The error bars indicate standard deviation (SD) (n=10) . The different letters identify a significant difference (Turkey HSD test, P<0.05)

DOI: 10.7287/peerj.preprints.27059v1/supp-6

Fig.5 Comparison of the population density of Wolbachia in T. confusum by different concentration of tetracycline treatment for 2, 3 and 4 weeks

The number expressed in terms of number of wsp copies per individual ( a: larvae CK=1.47E+07 , b: pupae CK: ♀ 2.22E+07 , ♂ 1.97E+07 , c: adults CK= 2.37E+07). The error bars indicate standard deviation (SD). The different letter indicates a significant difference (Turkey HSD test, P<0.05) .

DOI: 10.7287/peerj.preprints.27059v1/supp-7

raw data of the figures and tables

DOI: 10.7287/peerj.preprints.27059v1/supp-8

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Yujie Lu authored or reviewed drafts of the paper, approved the final draft.

Shiyuan Miao performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Zhengyan Wang conceived and designed the experiments.

Sibao Wang conceived and designed the experiments.

Chenguang Zhang analyzed the data, contributed reagents/materials/analysis tools.

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary files figures and tables.

Funding

This research was supported by National Natural Science Fund Project (No.31601890) and the Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology (No.2016XTCX01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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