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Molecular profiling of microbiota in human oral cavity, stomach and intestine

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Molecular profiling of microbiota in human oral cavity, stomach and intestine https://t.co/MvASskLnqe
Molecular profiling of microbiota in human oral cavity, stomach and intestine https://t.co/8MtQYq0IoO
12 days ago
Molecular profiling of microbiota in human oral cavity, stomach and intestine https://t.co/hn8th9g2HJ
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Supplemental Information

A Venn diagram of genera in the gut microbiota

DOI: 10.7287/peerj.preprints.27030v1/supp-1

Demographics of healthy people donating gastric juices

DOI: 10.7287/peerj.preprints.27030v1/supp-2

Statistics of sample sequence

DOI: 10.7287/peerj.preprints.27030v1/supp-3

Raw data of saliva 16S rRNA gene V4 sequences

The 16S rRNA gene V3-V5 region sequences of saliva microbiota were downloaded from Human Microbiome Project (HMP) and the V4 sequences were cut from the V3-V5 sequences by a program designed by Perl language.

DOI: 10.7287/peerj.preprints.27030v1/supp-4

Raw data of stool 16S rRNA gene V4 sequences, section 1

The 16S rRNA gene V3-V5 region sequences of stool microbiota were downloaded from Human Microbiome Project (HMP) and the V4 sequences were cut from the V3-V5 sequences by a program designed by Perl language.

DOI: 10.7287/peerj.preprints.27030v1/supp-5

Raw data of stool 16S rRNA gene V4 sequences, section 2

The 16S rRNA gene V3-V5 region sequences of stool microbiota were downloaded from Human Microbiome Project (HMP) and the V4 sequences were cut from the V3-V5 sequences by a program designed by Perl language.

DOI: 10.7287/peerj.preprints.27030v1/supp-6

Raw data of stomach juice 16S rRNA gene V4 sequences

The 16S rRNA gene V4 region of gastric juice microbiota was sequenced by Miseq platform.

DOI: 10.7287/peerj.preprints.27030v1/supp-7

Additional Information

Competing Interests

Colin K. He is a chief science officer (CSO) employed by Stegotech LLC (Audubon, USA). Jianwei Chen is a bioinformatic engineer employed by BGI-Qingdao (Qingdao, People’s Republic of China).

Author Contributions

Qianqian Liu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Feizhou Zhu conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Liyu Chen conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper.

Meihua Xu performed the experiments, authored or reviewed drafts of the paper.

Jianwei Chen contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper.

Colin K. He contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper.

Lingzhi Zou collected samples..

Na Jiang collected samples..

Xianghong Liu collected samples..

Yang Meng collected samples..

Shijing Liao collected samples..

Yong Peng authored or reviewed drafts of the paper, approved the final draft.

Lili Wang collected samples..

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The study was approved by the independent Ethics Committee of Xiangya Hospital of Central South University in accordance with the ethical guidelines of the Declaration of Helsinki (No. 201512548).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Raw data of 16S rRNA gene V4 sequences in this study had been submitted as supplemental files to be available during reviewing. Also, all 16S rRNA gene V4 sequences of gastric juice bacterial flora had been submitted to Sequence Read Archive (SRA) with accession number SRP139893 (the SRA submission will be released on 2019-04-02 or upon publication, whichever is first); All 16S rRNA gene V3-V5 region sequences of saliva and stool bacterial flora in this study are available in the HMP (https://www.hmpdacc.org/).

Data Deposition

The following information was supplied regarding data availability:

Raw data of 16S rRNA gene V4 sequences in this study had been submitted as supplemental files to be available during reviewing. Also, all 16S rRNA gene V4 sequences of gastric juice bacterial flora had been submitted to Sequence Read Archive (SRA) with accession number SRP139893 (the SRA submission will be released on 2019-04-02 or upon publication, whichever is first); All 16S rRNA gene V3-V5 region sequences of saliva and stool bacterial flora in this study are available in the HMP (https://www.hmpdacc.org/).

Funding

This work was supported by the Natural Science Foundation of Hunan Province, PR. China (No.2015jj2162), Science and Technology Plan Project of Hunan Province, China (No.2017SK2092), the Research Foundation for Independent Innovation of Graduate Students in Central South University (No.2017zzts359), the Institute Research Project of Hunan Province. China (No. 14C0879). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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