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High-throughput metabarcoding reveals the effect of physicochemical soil properties on soil and litter biodiversity and community turnover across Amazonia

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#metabarcoding #biodiversity Amazonian biodiversity patterns in soils https://t.co/J0bp5X2jb4 via @PeerJPreprints
86 days ago
High-throughput metabarcoding reveals the effect of physicochemical soil properties on soil and litter biodiversity and community turnover across Amazonia https://t.co/6BqW9XMbQ5
High-throughput metabarcoding reveals the effect of physicochemical soil properties on soil and litter biodiversity and community turnover across Amazonia https://t.co/PpGXCSpZ1r
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Supplemental Information

Supplementary material

Additional tables and figures from analyzed data.

DOI: 10.7287/peerj.preprints.27012v1/supp-1

Metadata

Soil, coordinates and locality information of data collected in this study.

DOI: 10.7287/peerj.preprints.27012v1/supp-2

Operational Taxonomic Units table for 16S used in this study

DOI: 10.7287/peerj.preprints.27012v1/supp-3

Operational Taxonomic Units table for 18S used in this study

DOI: 10.7287/peerj.preprints.27012v1/supp-4

Taxonomic assignment of 16S operational taxonomic units

DOI: 10.7287/peerj.preprints.27012v1/supp-5

Taxonomic assignment of 18S operational taxonomic units

DOI: 10.7287/peerj.preprints.27012v1/supp-6

Sequences of 18S produced in this study

DOI: 10.7287/peerj.preprints.27012v1/supp-7

Sequences of 16S produced in this study

DOI: 10.7287/peerj.preprints.27012v1/supp-8

Script of presence/absence analysis

DOI: 10.7287/peerj.preprints.27012v1/supp-9

Script for abundance analysis

DOI: 10.7287/peerj.preprints.27012v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Camila D Ritter conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Alexander Zizka analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Fabian Roger analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Hanna Tuomisto authored or reviewed drafts of the paper, approved the final draft.

Christopher Barnes authored or reviewed drafts of the paper, approved the final draft.

R. Henrik Nilsson conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Alexandre Antonelli conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

The sample collection was authorized by Brazilian authorities: ICMBio (registration number 48185-2) and IBAMA (registration number 127341).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The sequence are in supplementary material as 16S_otus.fa and 18S_otus.fa.

Data Deposition

The following information was supplied regarding data availability:

Table 16S OTUs: Table 18S OTUs; Table 16S taxonomic assignment; Table 18S taxonomic assignment; Table metadata; 16S fasta files; 18S fasta files.

The raw data are provided in the Supplemental Files.

Funding

This study had primary financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico - Brazil: 249064/2013-8) and the Swedish Research Council (B0569601). Antonelli is further supported by the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013, ERC Grant Agreement n. 331024), the Swedish Foundation for Strategic Research, a Wallenberg Academy Fellowship, the Faculty of Sciences at the University of Gothenburg, and the David Rockefeller Center for Latin American Studies at Harvard University. all the funding or sources of support (whether external or internal to your organization) received during this study is listed above. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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