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Supplemental Information

Primer sequence for qRT-PCR

DOI: 10.7287/peerj.preprints.27003v1/supp-1

The details of the Human Phospho-MAPK Array coordinates

DOI: 10.7287/peerj.preprints.27003v1/supp-2

KEGG pathway enrichment of differentially expressed proteins

DOI: 10.7287/peerj.preprints.27003v1/supp-3

The morphology of C17.2 cells treated with indicated concentrations of GDF11

Images were obtainedat 50Xmagnification by inverted fluorescence microscope. The live cells were stained with calcein AM in green, and the dead cells were stained with EthD-1 in red.

DOI: 10.7287/peerj.preprints.27003v1/supp-4

The overview of the 23 MAPKs and other serine/threonine kinases detected using human Phospho-MAPK array kit

DOI: 10.7287/peerj.preprints.27003v1/supp-5

raw data for statistical analysis

DOI: 10.7287/peerj.preprints.27003v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Zongkui Wang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Miaomiao Dou performed the experiments, approved the final draft.

Fengjuan Liu performed the experiments, approved the final draft.

Peng Jiang performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.

Shengliang Ye performed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

Li Ma analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.

Haijun Cao contributed reagents/materials/analysis tools, approved the final draft.

Xi Du performed the experiments, approved the final draft.

Pan Sun contributed reagents/materials/analysis tools, approved the final draft.

Na Su contributed reagents/materials/analysis tools, approved the final draft.

Fangzhao Lin contributed reagents/materials/analysis tools, approved the final draft.

Rong Zhang conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Changqing Li conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary files :raw data for statistical analysis, raw data for western blot, Table S2 and Table S3.

Funding

This work was supported by PUMC Youth Fund and the Fundamental Research Funds for the Central Universities (grant number 2017310036); CAMS Innovation Fund for Medical Sciences (grant number 2016-I2M-1-018 and 2017-I2M-3-021); and the Scientific Research Project of Sichuan Medical Association (grant number S17082). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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