Human primary T cells: A practical guide
- Published
- Accepted
- Subject Areas
- Cell Biology, Genetics, Molecular Biology, Immunology
- Keywords
- t cells, practical guide, tissue culture, primary cells, crispr, electroporation, activation, pbmc
- Copyright
- © 2018 Aksoy et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Human primary T cells: A practical guide. PeerJ Preprints 6:e26993v1 https://doi.org/10.7287/peerj.preprints.26993v1
Abstract
Human primary T cells are invaluable and feasible model systems to study the characteristics of the immune cells in various contexts, including but not limited to cancer immunotherapy. Following isolation of T cells from fresh human blood samples, it is possible to culture, expand, and manipulate these cells, which allows extensive investigation for research purposes. Techniques for isolation and handling of T cells are well-established but parts of the protocols can highly vary across different labs. These differences in the protocols are there due to historical reasons and are often only supported by anecdotal evidence. We systematically modified basic components of the T cell culturing protocols and collected data on how they altered the final yield. Here, based on these data, we provide practical hints and tips on basic cellular and molecular techniques for handling primary human T cells. We hope that this guide will serve as a reference point to allow evaluate, discuss, and improve current practices in T cell culturing and manipulation.
Author Comment
This is a preprint submission to PeerJ Preprints. A live version of this guide is available at https://bit.ly/tcellguide.