Seasonal changes in community composition and diversity of bacterioplankton in an artificial lagoon in China

Qing ying Lin; Bin Sun; Kai qiang Bai; Jian Yang; Pei min He

doi:10.7287/peerj.preprints.26935v1

Seasonal changes in community composition and diversity of bacterioplankton in an artificial lagoon in China

Qing ying Lin, Bin Sun , Kai qiang Bai, Jian Yang, Pei min He

School of Marine Ecology and Environment, Shanghai Ocean University, ShangHai, Shanghai, China

DOI: 10.7287/peerj.preprints.26935v1

Published: 2018-05-16
Accepted: 2018-05-16

Subject Areas: Marine Biology, Microbiology
Keywords: artificial lagoon, bacterioplankton community, Illumina, environment

Copyright: © 2018 Lin et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Lin Qy, Sun B, Bai Kq, Yang J, He Pm. 2018. Seasonal changes in community composition and diversity of bacterioplankton in an artificial lagoon in China. PeerJ Preprints 6:e26935v1 https://doi.org/10.7287/peerj.preprints.26935v1

Abstract

Coastal lagoons are highly productive ecosystems. However, coastal lagoons are experiencing the effects of human disturbances at an increasing rate. Bacteria are key ecological players within lagoons, yet little is known about the magnitude, patterns, and drivers of diversity in these transitional environments. In this report, a seasonal study in the Fengxian artificial lagoon (China) was conducted, along with the adjacent sea, to simultaneously explore diversity in different domains and their spatio-temporal variability. Bacterioplankton community structures of surface waters from four sites over the course of four seasons were characterized with Illumina platform sequencing technology. The results showed significant differences in bacterioplankton communities between the four sites. In addition, the results indicated a difference between the enclosed lagoon and offshore waters during the same seasons. Seasonality was shown to be more important than spatial variability in shaping assemblages. The community barplot showed that, with the exception of January which had the same dominant genus in both the enclosed lagoon and offshore water, all other seasons had different genus. Likewise, the heatmap showed that the largest dissimilarity of bacterial species diversity occurred in July between the enclosed lagoon and offshore water, while the highest similarity was in January. This result paralleled genetic studies which also showed that gene expression had not only similar seasonal but spatial changes. Canonical correspondence analysis (CCA) analysis of all water samples showed that environmental indicators of dissolved oxygen, temperature, PO₄, NO₂, and Chemical Oxygen Demand (COD) contributed to the variation. Bacterial communities in the lagoon are affected by temperature, dissolved oxygen and NO₂, while the dominant bacterial communities offshore are affected by COD and PO₄. This study provided evidence for a temporally dynamic structure of bacterial assemblages in lagoons. In this vulnerable ecosystem, there is interplay of seasonally-influenced environmental drivers that works together to shape bacterial assemblages. The goal is to identify beneficial microbial population that improved water quality in an enclosed lagoon in order to provide a new perspective for optimizing the breeding environment.