RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)
- Published
- Accepted
- Subject Areas
- Agricultural Science, Developmental Biology, Entomology
- Keywords
- Leguminivora glycinivorella, immune-related gene, RNA interference, double-stranded RNA, artificial feeding assay
- Copyright
- © 2018 Ran et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae) PeerJ Preprints 6:e26923v2 https://doi.org/10.7287/peerj.preprints.26923v2
Abstract
RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNAi-mediated SPB control methods.
Author Comment
we want to show founding support on this article. Meanwhile, Figture 5 have some mistake and we have revised them.
Supplemental Information
Table S1. Details regarding the immune-related genes ofLeguminivora glycinivorella
Table S2. Primers used for synthesising dsRNA and analysing candidate genes
Fig S1. Phylogenetic relationships among gram-negative–binding proteins (GNBPs) from Leguminivora glycinivorella and Drosophila melanogaster.
Fig S2. Predicted Leguminivora glycinivorella clip-domain serine proteases (CLIPs)
Fig S3. Alignment of multiple C-type
Alignment of multiple C-type (A) and I-type (B) Leguminivora glycinivorella lysozyme sequences. ClustalX was used to align sequences.
Raw data of Table S1
Nucleic acid sequence of the immune-related genes of L. glycinivorella obtained in the study.
Raw data of Table S1
Protein sequence of the immune-related genes of L. glycinivorella obtained in the study.