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RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

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RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae) https://t.co/k4jGY6ugVo RNA interference (RNAi) technology may be useful for developing new crop protection s…
820 days ago
RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae) https://t.co/9xuuU7LHsS
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Supplemental Information

Table S1. Details regarding the immune-related genes ofLeguminivora glycinivorella

DOI: 10.7287/peerj.preprints.26923v2/supp-1

Table S2. Primers used for synthesising dsRNA and analysing candidate genes

DOI: 10.7287/peerj.preprints.26923v2/supp-2

Fig S1. Phylogenetic relationships among gram-negative–binding proteins (GNBPs) from Leguminivora glycinivorella and Drosophila melanogaster.

DOI: 10.7287/peerj.preprints.26923v2/supp-3

Fig S2. Predicted Leguminivora glycinivorella clip-domain serine proteases (CLIPs)

DOI: 10.7287/peerj.preprints.26923v2/supp-4

Fig S3. Alignment of multiple C-type

Alignment of multiple C-type (A) and I-type (B) Leguminivora glycinivorella lysozyme sequences. ClustalX was used to align sequences.

DOI: 10.7287/peerj.preprints.26923v2/supp-5

Raw data of Table S1

Nucleic acid sequence of the immune-related genes of L. glycinivorella obtained in the study.

DOI: 10.7287/peerj.preprints.26923v2/supp-6

Raw data of Table S1

Protein sequence of the immune-related genes of L. glycinivorella obtained in the study.

DOI: 10.7287/peerj.preprints.26923v2/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ruixue Ran performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft, ruixueRan was photographer of figure 5 (Images of larvae fed an artificial diet supplemented with dsRNA).

Tianyu Li performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Xinxin Liu analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Hejia Ni performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Wenbin Li contributed reagents/materials/analysis tools.

Fanli Meng conceived and designed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw data/code is included in the manuscript.

Funding

This work was supported by the Foundation for University Key Teacher of the Department of Education of Heilongjiang Province (1253G008). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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