Characterization and comparison of the intestinal microbiota in Chinese Shanxi black pig during the weaning and nursery periods

Pengfei Gao; Haizhen Wang; Zhimin Cheng; Yuanyuan Wang; Pengkang Song; Xiaohong Guo; Zhibian Duan; Guoqing Cao; Jianfeng Liu; Min Du; Bugao Li

doi:10.7287/peerj.preprints.26893v1

Characterization and comparison of the intestinal microbiota in Chinese Shanxi black pig during the weaning and nursery periods

Pengfei Gao¹, Haizhen Wang¹, Zhimin Cheng¹, Yuanyuan Wang¹, Pengkang Song¹, Xiaohong Guo¹, Zhibian Duan¹, Guoqing Cao¹, Jianfeng Liu², Min Du³, Bugao Li ¹

1 Department of Animal Sciences and Veterinary Medicine, Shanxi Agricultural University, Taigu, China

2 College of Animal Science and Technology, China Agricultural University, Beijing, China, China

3 Department of Animal Sciences, Washington State University, Pullman, WA, USA

DOI: 10.7287/peerj.preprints.26893v1

Published: 2018-04-26
Accepted: 2018-04-26

Subject Areas: Biodiversity, Microbiology
Keywords: 16S rRNA, Functional capacity, Gut microbiota, Weaning and nursery periods, Chinese Shanxi Black pigs

Copyright: © 2018 Gao et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Gao P, Wang H, Cheng Z, Wang Y, Song P, Guo X, Duan Z, Cao G, Liu J, Du M, Li B. 2018. Characterization and comparison of the intestinal microbiota in Chinese Shanxi black pig during the weaning and nursery periods. PeerJ Preprints 6:e26893v1 https://doi.org/10.7287/peerj.preprints.26893v1

Abstract

Background. The intestinal microbiota plays essential functions that affect piglet health at the weaning stage. However, our understanding of the succession of bacterial intestinal communities during weaning and Nursery Periods of pig is limited.

Methods. To better understand this, we performed 16S rDNA gene sequencing of the contents of four distinct intestinal segments [duodenum (D), jejunum (J), ileum (I), and cecum (C)] in six Chinese Shanxi Black pigs at day 25 (B25 group; i.e., weaning) and day 70 (B70 group; i.e., nursery).

Results. We found that the dominant phyla are Proteobacteria, Bacteroidetes, and Firmicutes. In addition, the dominant genera were Acinetobacter, Prevotella, Streptococcus, and SMB53 (Clostridiaceae). The microbiota in the B25 group across segments were significantly different from that in the corresponding segments in the B70 group. In addition, the distinct segments in the B70 group presented a continuum consisting of compartmentalized architecture whereas the distinct segments in B25 group were relatively similar. The predicted molecular function analysis revealed higher enrichments in associated metabolisms as well as stress-induced functions in the B25 group.

Conclusions. This study provides insights into the succession of intestinal microbiota in Chinese Shanxi Black pigs before and after weaning, and provides reference for improving the intestinal development of piglets.