Water contaminated with Didymosphenia geminata alters fish cell line viability
- Published
- Accepted
- Subject Areas
- Biotechnology, Freshwater Biology, Ecohydrology
- Keywords
- Didymo, Cells line, viability, Toxicology
- Copyright
- © 2018 Olivares et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Water contaminated with Didymosphenia geminata alters fish cell line viability. PeerJ Preprints 6:e26880v2 https://doi.org/10.7287/peerj.preprints.26880v2
Abstract
Didymosphenia geminata (D. geminata) in Chilean rivers is a complex problem. Its biology and effects on ecosystems is still being studied, at the moment not research has focused on its D. geminata effects at the cellular level. We developed an artificial river system to maintain D. geminata study material and evaluate effects of water contaminated with this diatom on the viability of two fish cell lines. Results indicate that CHSE-214 cells are sensitive to increasing D. geminata extract concentrations, reducing crop viability by 50% when exposed for 24 hours at a 0.01V/V dilution and reducing proliferative capacity by 70% on a 5 day temporal curve. SHK-1 cells showed lower sensitivity, presenting a decrease of 20% in viability at 24 hours, and a lower cell proliferation rate by day 5, but higher than of the CHSE-214 cells. Both lines were affected by exposure to D. geminata extracts, but CHSE-214 lines were more sensitive to polyphenols extracted from the microalgae. We conclude that certain cell types are sensitive to D. geminata in rivers, meaning that chronic effects on aquatic species contaminated with this diatom should be observed. Effects of this plague at a cellular level can be further studied to understand its full impact on river ecosystems.
Author Comment
This is a preprint submission to PeerJ Preprints. We improve the affiliation and details of methods
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