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Supplemental Information

OTU table

OTUs were filtered to 0.1% in any sample.

DOI: 10.7287/peerj.preprints.2681v1/supp-1

Shannon's diversity index and read counts for sample groups

Shannon's diversity and read counts are not different between birth weight or diet groups.

DOI: 10.7287/peerj.preprints.2681v1/supp-2

Shannon's diversity of every sample

Output from diversity function in R (Vegan 2.3-2) performed on OTU table.

DOI: 10.7287/peerj.preprints.2681v1/supp-3

Compositional biplot

The samples are coloured according to diet and birth weight groups. The biplot is drawn to show the relationship between the OTUs [scale = 1].

DOI: 10.7287/peerj.preprints.2681v1/supp-4

ALDEx2 table

Output from ALDEx2 test for significantly different OTUs between diet groups.

DOI: 10.7287/peerj.preprints.2681v1/supp-5

ALDEx2 test heat map

OTUs divergent between diet groups by Wilcoxon rank-sum test are shown. Colour corresponds to the log2 of the OTUs’ abundance.

DOI: 10.7287/peerj.preprints.2681v1/supp-6

Scripts utilized to generate figures

R scripts are provided here to generate figures and data 1, 2, S2, S3, S4, S5, S6 from the provided OTU table (S1)

DOI: 10.7287/peerj.preprints.2681v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Kait Al conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Ousseynou Sarr performed the experiments, analyzed the data, reviewed drafts of the paper.

Kristyn Dunlop reviewed drafts of the paper.

Gregory B Gloor analyzed the data, reviewed drafts of the paper.

Gregor Reid contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Jeremy Burton conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Timothy RH Regnault conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The University of Western Ontario Animal Use Subcommittee approved all procedures, AUP Number: 2010-229. Animal care, maintenance, and surgeries were conducted in accordance with the standards set by the Canadian Council on Animal Care.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary file.

Funding

Funding was provided by the Canadian Institutes of Health Research (#MOP-209113) and the W. Garfield Weston Foundation. OS was supported by the Whaley Postdoctoral Fellowship.  The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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