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Supplemental Information

The base peak of digest

(A) C-entolimod is digested by trypsin (T). (B) C-entolimod is digested by chymotrypsin (CT).

The sequence coverage by T digest is

1 CFNHMWSHPQ FEKAQVINTN SLSLLTQNNL NKSQSSLSSA IERLSSGLRI 51 NSAKDDAAGQ AIANRFTSNI KGLTQASRNA NDGISIAQTT EGALNEINNN 101 LQRVRELSVQ ATNGTNSDSD LKSIQDEIQQ RLEEIDRVSN QTQFNGVKVL 151 SQDNQMKIQV GANDGETITI DLQKIDVKSL GLDGFNVNSP GISGGGGGIL 201 DSMGTLINED AAAAKKSTAN PLASIDSALS KVDAVRSSLG AIQNRFDSAI 251 TNLGNTVTNL NSARSRIEDA DYATEVSNMS KAQILQQAGT SVLAQANQVP 301 QNVLSLLRHH HHHHGS

Coverage: 98% Score: 106137

The sequence coverage by CT digest is

1 CFNHMWSHPQ FEKAQVINTN SLSLLTQNNL NKSQSSLSSA IERLSSGLRI 51 NSAKDDAAGQ AIANRFTSNI KGLTQASRNA NDGISIAQTT EGALNEINNN 101 LQRVRELSVQ ATNGTNSDSD LKSIQDEIQQ RLEEIDRVSN QTQFNGVKVL 151 SQDNQMKIQV GANDGETITI DLQKIDVKSL GLDGFNVNSP GISGGGGGIL 201 DSMGTLINED AAAAKKSTAN PLASIDSALS KVDAVRSSLG AIQNRFDSAI 251 TNLGNTVTNL NSARSRIEDA DYATEVSNMS KAQILQQAGT SVLAQANQVP 301 QNVLSLLRHH HHHHGS

Coverage: 91% Score: 8248

Combine T and CT, the coverage is 100%.

DOI: 10.7287/peerj.preprints.26803v1/supp-1

Dual-luciferase reporter assay

DOI: 10.7287/peerj.preprints.26803v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Bingyu Ye conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Wenlong Shen analyzed the data.

Minglei Shi contributed reagents/materials/analysis tools.

Yan Zhang contributed reagents/materials/analysis tools.

Cunshuan Xu conceived and designed the experiments, contributed reagents/materials/analysis tools.

Zhihu Zhao conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All animal experiments with the ethical approval of the Beijing Institute of Biotechnology.

Funding

This research was supported by the National Basic Research Program of China (2013CB966802), National Natural Science Foundation of China (31370762, 31030026, 31071119, 81372218) and National Key Technology R&D Program of China (2012BAI01B07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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