Evaluation of buriti endocarp as lignocellulosic substrate for second generation ethanol production

Instituto de Ciência e Tecnologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brasil
Programa de Pós-Graduação em Biocombustíveis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brasil
Departamento de Ciências Básicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brasil
DOI
10.7287/peerj.preprints.26777v1
Subject Areas
Agricultural Science, Bioengineering, Food, Water and Energy Nexus
Keywords
Mauritia flexuosa, pre-treatment, saccharification, bioethanol
Copyright
© 2018 Rodrigues et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Rodrigues PR, Araújo MF, Rocha TL, Veloso RVS, Pantoja LA, Santos AS. 2018. Evaluation of buriti endocarp as lignocellulosic substrate for second generation ethanol production. PeerJ Preprints 6:e26777v1

Abstract

The production of lignocellulosic ethanol is one of the most promising alternatives to fossil fuels, however, this technology still faces many challenges related to the viability of the alcohol in the market. In this paper the endocarp of buriti fruit was assessed for ethanol production. The whole fruit was characterized physically and chemically and its endocarp submitted to acid and alkaline pre-treatments, which were optimized through the use of surface response methodology for removal of hemicellulose and lignin, respectively. Hemicellulose content was reduced by 88% after acid pretreatment. Alkaline pre-treatment reduced the lignin content in the recovered biomass from 11.8% to 4.2% and increased the concentration of the cellulosic fraction to 88.5%. The pre-treated biomass was saccharified by the action of cellulolytic enzymes and, in the optimized condition, was able to produce 110 g of glucose per L of hydrolyzate. Alcoholic fermentation of the enzymatic hydrolyzate bio-catalized by Saccharomyces cerevisiae resulted in a fermented medium with 4.3% ethanol and YP/S of 0.33.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Fermentation of pretreated and hydrolyzed buriti endocarp, raw data

DOI: 10.7287/peerj.preprints.26777v1/supp-1