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Supplemental Information

Raw enzyme activity assay values

Activity values are displayed in mmols/min/mg protein for CAT and nmols/min/mg protein for GR, GPx, and SOD. Assays were rerun if replicates experienced errors, these could be due in part to issues such as bubbling during the metabolism of substrates. If assays could not be rerun due to concerns about sample or reagent integrity, those values were excluded (-).

DOI: 10.7287/peerj.preprints.26765v1/supp-1

Untruncated western blotting image of Superoxide dismutase (23 kDa) versus moon phase cycle

Samples are denoted by arrows.

DOI: 10.7287/peerj.preprints.26765v1/supp-2

Acute tracking of SOD versus time following peak reproduction (¼ moon phase)

Samples labeled by arrows denoting collection time points following peak reproduction.

DOI: 10.7287/peerj.preprints.26765v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

James WA Murphy conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Abby C Collier analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Robert H Richmond contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Department of Land and Natural Resources - Division of Aquatic Resources coral collection permit 2015-06 and 2015-17 (O‘ahu, HI, USA).

Funding

This project was financially supported through National Oceanic and Atmospheric Administration (grant number NA09NOS4780178) and the Charles H. and Margaret B. Edmondson Research Fund (University of Hawai‘i at Mānoa Department of Biology). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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