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Novel, non-symbiotic isolates of Neorhizobium from a dryland agricultural soil

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1148 days ago
Novel, non-symbiotic isolates of Neorhizobium from a dryland agricultural soil https://t.co/KHFWNb1O1d
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Supplemental Information

Schematic representation of the semi-selective enrichment procedure to isolate fast-growing Rhizobia from the Tomejil soil

DOI: 10.7287/peerj.preprints.26724v1/supp-1

PCR amplification of a genomic DNA band from Tomejil Neorhizobium sp. strains with fnrN primers

(A) Agarose gel electrophoresis separation of PCR products. (B) Schematic representation of the genomic region amplified by fnrN primers in Tomejil Neorhizobium sp. strains (see text).

DOI: 10.7287/peerj.preprints.26724v1/supp-2

Physicochemical properties of Tomejil soil

DOI: 10.7287/peerj.preprints.26724v1/supp-3

GenBank IDs of DNA sequences obtained or used in this work

DOI: 10.7287/peerj.preprints.26724v1/supp-4

Pairwise Average Nucleotide Identities (ANIb) between representative Neorhizobium sp. Tomejil isolates and Neorhyzobium sp. type strains

DOI: 10.7287/peerj.preprints.26724v1/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Amalia Soenens conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Juan Imperial conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The 16S rDNA and rpoB sequences described here were deposited in GenBank under accession numbers MG957216 to MG957235, and MG966473 toMG966492, respectively. Genome sequences were deposited in GenBank under Bioprojects

Data Deposition

The following information was supplied regarding data availability:

No raw data / code was generated

Funding

This work was supported by the CONSOLIDER-INGENIO program (CSD2009-00006) and by project LUPIGEN (CGL2011-26932), both from the Spanish Plan Nacional de Investigación Científica y Técnica. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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