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Identification of the Telomere elongation mutation in Drosophila

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RT @fly_papers: Identification of the Telomere elongation mutation in Drosophila https://t.co/3EeBX9CCfU
RT @fly_papers: Identification of the Telomere elongation mutation in Drosophila https://t.co/3EeBX9CCfU
191 days ago
Identification of the Telomere elongation mutation in Drosophila https://t.co/3EeBX9CCfU
191 days ago
Identification of the Telomere elongation mutation in Drosophila https://t.co/49rkHLgRQn
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Supplemental Information

Figure S1 Telomere length in recombinants from transposons used for Tel1 mapping

Change in relative HeT-A copy number in different recombinant stocks from generation 0 to 12. Recombinants carrying ca are shown in purple; those bearing st in red.

DOI: 10.7287/peerj.preprints.26708v1/supp-1

FlyBase browser map of the 77kb Tel1 region with indels and Minos insertion sites

Genome browser map for the 77 kb region between 05151 and d10097 (red vertical lines). Blue arrows show the location of two candidate indels (deletion C and deletion TGT). Two transposon insertions, MI03112 and MI02316, which lie between the two indels, were used for further mapping the Tel1 mutation. Red arrow indicates the MB09416 transposon insertion site. Flies carrying this insertion have long telomeres.

DOI: 10.7287/peerj.preprints.26708v1/supp-2

Sequence conservation among insect species at Tel1 and MB09416 insertion loci

UCSC Genome Browser maps showing conservation in the immediate vicinity of the Tel1 mutation (deletion TGT) and the MB09416 insertion. The sequence around the Tel1 mutation shows good conservation among different Drosophila and insect species, whereas the sequence surrounding the MB09416 insertion site is not well conserved.

DOI: 10.7287/peerj.preprints.26708v1/supp-3

PCR with flanking primers to large indels found in Tel genome

PCR products spanning two large indels 145 bp (3R: 15,157,140 – 284) and 85 bp (3R:15,170,445-524) from Tel and y w. These indels were identified in the Tel1 genome by manual scanning the CLC Genomics assembly. PCR product of 145 bp deletion shows a band size difference of ~40 bp, whereas 85 bp deletion shows expected band size variation.

DOI: 10.7287/peerj.preprints.26708v1/supp-4

Genome sequencing and analysis of large indels

DOI: 10.7287/peerj.preprints.26708v1/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Hemakumar M. Reddy conceived and designed the experiments, performed the experiments, analyzed the data.

Thomas A. Randall conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Radmila Capkova Frydrychova performed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

James M. Mason conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

BioProject Accession PRJNA255315 at NCBI

Funding

This work was supported by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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