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Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Diana Madeira performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

José Eduardo Araújo performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Rui Vitorino analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Pedro Costa analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Catarina Vinagre conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Mário Sousa Diniz conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

This study was carried out in accordance with the recommendations of EU legislation for animal experimentation (Directive 2010/63/EU). The protocol was approved by the Direcção Geral de Alimentação e Veterinária.

Data Deposition

The following information was supplied regarding data availability:

Raw data has already been published.

Funding

This study had the support of the Portuguese Fundação para a Ciência e a Tecnologia (FCT) [individual grants: senior researcher position to CV, SFRH/BD/80613/2011 and SFRH/BPD/117491/2016 to DM; project grant PTDC/MAR/119068/2010 and strategic project grants UID/Multi/04378/2013, UID/MAR/04292/2013, UID/BIM/04501/2013, UID/IC/00051/2013, and UID/AMB/50017]. It also had the support of ERDF under the PT2020 Partnership Agreement and Compete 2020 [POCI-01-0145-FEDER-007728 and POCI-01-0145-FEDER-007638]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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