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Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding

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Interested how important sequencing different genes can change you metabarcoding results? @PeerJPreprints https://t.co/cRgDIScZgG #AquacultureFisheriesandFishScience #Bioinformatics #Genetics #metabarcoding #molecularbiology #NZscampi #COI #18SrRNA #MarineBiology
RT @aimz_vdReis: A prelim of what NZ scampi eat - @PeerJPreprints https://t.co/cRgDIScZgG #AquacultureFisheriesandFishScience #Bioinformat…
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Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding https://t.co/rWWqQAEjhA
Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding https://t.co/TAhMifg9kr
RT @aimz_vdReis: A prelim of what NZ scampi eat - @PeerJPreprints https://t.co/cRgDIScZgG #AquacultureFisheriesandFishScience #Bioinformat…
A prelim of what NZ scampi eat - @PeerJPreprints https://t.co/cRgDIScZgG #AquacultureFisheriesandFishScience #Bioinformatics #Genetics
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Supplemental Information

Confidence levels for assigned taxa to OTUs for COI sequences

DOI: 10.7287/peerj.preprints.26667v1/supp-1

Confidence levels for assigned taxa to OTUs for 18S sequences

DOI: 10.7287/peerj.preprints.26667v1/supp-2

COI sequences from scampi digesta matching genus/species level

DOI: 10.7287/peerj.preprints.26667v1/supp-3

18S sequences from scampi digesta matching genus/species level

DOI: 10.7287/peerj.preprints.26667v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Aimee L van der Reis conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Olivier Laroche contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Andrew G Jeffs conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, logistics and administration.

Shane D Lavery conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Specimens for this study were collected in accordance with approvals under New Zealand’s Animal Welfare Act 1991. The transport and holding of the scampi, as well as the experimental procedures, were approved by the Animal Ethics Committee of the Nelson Marlborough Institute of Technology (AEC2014-CAW-02).

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

New Zealand’s Ministry of Primary Industries - Special Permit #549

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The sequences of the scampi diet matching genus/species have been uploaded as a supplemental file. Table S3 are the COI sequences and Table S4 are the 18S sequences.

Data Deposition

The following information was supplied regarding data availability:

Figshare

https://figshare.com/s/7724ecca36796209abf4

Funding

This study is part of ‘Ka Hao te Rangatahi: Revolutionary Potting Technologies and Aquaculture for Scampi’ (CAWX1316) funded by the New Zealand Ministry of Business Innovation and Employment. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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