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Supplemental Information

Supplementary methods and results

DOI: 10.7287/peerj.preprints.26623v1/supp-1

GenBank numbers and locality data for samples used in this study

DOI: 10.7287/peerj.preprints.26623v1/supp-2

BEAST MCC time trees for all seven focal species/lineages

Gray bars represent the upper and lower 95% highest posterior density (HPD) estimates of divergence times at each node and correspond to x-axis scale bars in units of millions of years ago (Ma). Yellow portions of node bars extend beyond x-axis limits and thus represent scale-less, truncated estimates; for upper 95% HPD ends of the truncated bars, and for nodes corresponding to the WPI break, geometric mean divergence time estimate values are given. Tip labels are colored green (west of the WPI break) or purple (east of the WPI break) consistent with the colors of sampling locations in Fig. 2.

DOI: 10.7287/peerj.preprints.26623v1/supp-3

Graphical checks on prior distributions listed in Table 1 based on principal components analysis

Red dots show the observed data for (A) frogs and (B) freshwater fishes projected into the principal components space.

DOI: 10.7287/peerj.preprints.26623v1/supp-4

GIS sea-level model for lower Central America

This map is based on a 250 m Digital Elevation Model (DEM) and shows predicted eustatic sea levels in the study area potentially resulting from highstands of +50 m (light blue), +100 m (blue), and +250 m (dark blue) above present sea level (relative to current elevation).

DOI: 10.7287/peerj.preprints.26623v1/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Justin C Bagley conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Michael J Hickerson performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Jerald B Johnson conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Bagley, Justin; Hickerson, Michael (2018) Data for: Testing hypotheses of diversification in Panamanian frogs and freshwater fishes using hierarchical approximate Bayesian computation with model averaging [Data Set]. Mendeley Data, v2. Available at:


Justin C. Bagley was funded in part by a Graduate Research Fellowship from BYU Graduate Studies, and by a National Science Foundation (NSF) Doctoral Dissertation Improvement Grant (DEB-1210883; to Jerald B. Johnson and Justin C. Bagley). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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