"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.
April 4, 2018:[Expression of Concern] The publisher has been made aware of an authorship dispute with this preprint. We are investigating and will update this Expression of Concern as we get clarity on the situation.
Endogenous expression of thermo-sensitive ion channels TRPV1 and TRPV4 in immune tissues of avian species (duck, Anas platyrhynchos)
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Majhi RK, Maity A, Senapati MR, Behera PC, Mandal AK, Giri SC, Goswami C-.2018. Endogenous expression of thermo-sensitive ion channels TRPV1 and TRPV4 in immune tissues of avian species (duck, Anas platyrhynchos)PeerJ Preprints6:e26614v1https://doi.org/10.7287/peerj.preprints.26614v1
Calcium signaling and body temperature are two important factors governing activation of immune cells. However, molecular identities of major players involved in such critical regulations are still unknown, especially in avian systems. In this work we explored the endogenous expression of Transient Receptor Potential Vanilloid subtype 1 and subtype 4 (TRPV1 and TRPV4) channels, in the immune cells and different tissues of duck (Anas platyrhynchos). TRPV1 and TRPV4 represent two important non-selective ion channels which are also thermo sensitive in nature. Using confocal microscopy we demonstrate that TRPV1 and TRPV4 are expressed endogenously in the duck immune system such as thymus, spleen, caecum and bursa. In thymus, bursa of Fabricius and caecum, these channels are differentially localized in the plasma membrane and intra-cellular regions. In addition, using Flow cytometry we demonstrate very specific expression of these two channels in Peripheral Blood Mononuclear Cells (PBMCs) and CD3+ve T cells. This result is the first report of endogenous expression of TRPV channels in cells and tissues relevant for avian immune system. Such findings may have importance in the context of immune functions and responses to pathological challenges faced by birds. Presence of these two important ion channels in the duck immune system may also have commercial importance in the context of livestock management, food supplement and production of quality poultry products.
A large number of work has been done on mammalian immune system. However, not much work has been done on the avian immune system. In this pre-print we submit the expression of thermosensitive ion channels in specific tissue and cells that are part of avian immune system.