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Increased chemical acetylation of peptides and proteins in rats after daily ingestion of diacetyl analyzed by Nano-Lc-MS/MS

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RT @ecarrilh: Congrats Nilson, our preprint has been published today in @PeerJPreprints https://t.co/4QrEgYmXiI #Biochemistry #Toxicology
Congrats Nilson, our preprint has been published today in @PeerJPreprints https://t.co/4QrEgYmXiI #Biochemistry #Toxicology
201 days ago
Increased chemical acetylation of peptides and proteins in rats after daily ingestion of diacetyl analyzed by Nano-Lc-MS/MS https://t.co/0nAp43hT3M
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A peer-reviewed article of this Preprint also exists.

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Supplemental Information

NanoHPLC gradient elution

NanoHPLC gradient elution, gradient mobile phase consisting of (A) formic acid/ACN/ H2O (1:20:980) and (B) formic acid/ACN/ H2O (1:950:50)

DOI: 10.7287/peerj.preprints.26613v1/supp-1

Central residue H acetylated (control group)

Motif Analyse of Lung from control group.Central residue H acetylated

DOI: 10.7287/peerj.preprints.26613v1/supp-2

Central residue H acetylated in group treated with 540 mg/Kg/day of Diacetyl

Motif Analyse of Lung from group treated with 540 mg/Kg/day of Diacetyl. Central residue H acetylated

DOI: 10.7287/peerj.preprints.26613v1/supp-3

Central residue K acetylated in group treated with 540 mg/Kg/day of Diacetyl

Motif Analyse of Lung from group treated with 540 mg/Kg/day of Diacetyl. Central residue K acetylated

DOI: 10.7287/peerj.preprints.26613v1/supp-4

Central residue K acetylated in control group

Motif Analyse of Lung from control group. Central residue K acetylated

DOI: 10.7287/peerj.preprints.26613v1/supp-5

Central residue R acetylated in group treated with 540 mg/Kg/day of Diacetyl

Mottif Analyse of Lung from group treated with 540 mg/Kg/day of Diacetyl. Central residue R acetylated

DOI: 10.7287/peerj.preprints.26613v1/supp-6

Central residue R acetylated in control group

Motif Analyse of Lung from control group. Central residue R acetylated

DOI: 10.7287/peerj.preprints.26613v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests

Author Contributions

Leticia Dias Lima Jedlicka conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Sheila Barreto Guterres conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Aleksandro Martins Balbino performed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Giuseppe Bruno Neto performed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Richardt Gama Landgraf conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Liliam Fernandes conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Emanuel Carrilho conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Etelvino José Henriques Bechara conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Nilson A Assuncao conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

This study was approved by the Ethical Committee of the School Medicine of the Federal University of São Paulo

Data Deposition

The following information was supplied regarding data availability:

Data generated and analyzed during this study are included in this published article (and in supplementary information files) or are available in the [PRIDE] repository, https://www.ebi.ac.uk/pride/archive/

The details to deposit in PRIDE is:

Project Name: Wistar rat, lung, NanoLC-MS/MS

Project accession: PXD004504

Project DOI: Not applicable

https://www.ebi.ac.uk/pride/archive/projects/PXD004504

Reviewer account details:

Username: reviewer14062@ebi.ac.uk

Password: S4pJvG0F

Funding

This material is based upon work supported by São Paulo Research Foundation (FAPESP, grants no. 2012/02514-9, 2013/07763-0, and 2010/01404-0) and Brazilian Innovation Agency (FINEP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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