Nowadays, with the development of people's life, obesity become one of the largest health problems today. Solute carrier family 35, member D3 (SLC35D3) protein has been reported to be involved in adipose deposition and metabolic control in mice. Because organ size in pig is comparable to that of the human, the pig is an ideal model that has been used to study diabetes mellitus, atherosclerosis, obesity, and other diseases. To better understand the structure and function of the SLC35D3 gene, the Meishan pig SLC35D3 gene was cloned and characterized, and its expression in different tissues was determined. The SLC35D3 cDNA consisted of a 1272 bp coding sequence that encoded a protein of 243 amino acids with a molecular mass of 44653.9 Da, and 966 bp 3′ untranslated regions. It is a pity that we have not got 5′ untranslated regions. Phylogenetic tree analysis revealed that porcine SLC35D3 had a closer genetic relationship and a shorter evolutionary distance with Vicugna; however, its evolutionary distance with that of the gorilla was longer than that of Vicugna. In addition, we quantified the SLC35D3 mRNA level by real-time polymerase chain reaction and detected expression in the liver, kidney, lung, heart, brain, LM, and spleen, as well as in the leaf lard, SAT, and PAT. In addition, we also tested the expression level of Meishan, Bama and Yorkshire in adipose tissue. The SLC35D3 mRNA level was highest in the leaf lard. These results serve as a foundation for further study on the porcine SLC35D3 gene.