Differences in expression density and molecular weight of CR1-Like in erythrocytes of landrace swine

College of Animal Science and Technology, Shanxi Agricultral University, Taigu, Shanxi, China
DOI
10.7287/peerj.preprints.26596v1
Subject Areas
Agricultural Science, Immunology
Keywords
CR1-like on porcine erythrocytes, Expression level, Molecular weight, Differences
Copyright
© 2018 Zhang et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Zhang J, Yin W, Wang C, Jia R, Fan K, Sun N, Sun Y, Li H. 2018. Differences in expression density and molecular weight of CR1-Like in erythrocytes of landrace swine. PeerJ Preprints 6:e26596v1

Abstract

Background. Porcine erythrocytes express complement receptor 1-like (CR1-like), which is involved in immune adherence.

Methods. In this study, porcine erythrocyte samples were collected from fifty-five individual Landrace swine to characterize differences in porcine CR1-like. Flow cytometry analysis was performed to examine the porcine differences in CR1-like expression density and immunoprecipitation, SDS-PAGE and Western blot were performed to detect variations in porcine CR1-like molecular weights.

Results. Different mean fluorescence intensities (MFI) of porcine erythrocytes were identified in three groups as 33.016±2.889 (40.0%), 59.974±9.299 (45.5%) and 131.241±8.375 (14.5%). Under reduced condition, three porcine CR1-like molecular weight variants were identified as 85.280±0.935 kDa (9.09%), 123.939±2.752 kDa (14.55%) and 136.696±2.028 kDa (76.36%).

Discussion. CR1-like was dispersed on the surface of porcine erythrocytes and promoted immune adherence. There have been no reports on whether differences in the expression levels and/or molecular weights of CR1-like in erythrocytes represent diversity in different individuals, and if so, whether this diversity influences the immune adherence of erythrocytes and/or whether the diversity is associated with CR1-like polymorphisms. At present, five candidate genes that are related to the differences above were found. Research examining erythrocyte immune adherence and CR1-like genes is under way. These results will provide theoretical data for future studies of the immunological mechanism of CR1-like in porcine erythrocytes.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Differences in Expression Density and Molecular Weight of CR1-Like in Erythrocytes of Landrace Swine

Table 1 and Table 2 are the raw data for the expression level and molecular weights of CR1-like on porcine erythrocytes.

DOI: 10.7287/peerj.preprints.26596v1/supp-1