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Shumkova R, Neov B, Sirakova D, Georgieva A, Teofanova D, Radoslavov G, Bouga M, Hristov P.2018. The first molecular detection and phylogenetic assessment of six honeybee viruses in Apis mellifera L. colonies in Bulgaria. PeerJ Preprints6:e26595v1https://doi.org/10.7287/peerj.preprints.26595v1
Honey bee colonies suffer from various pathogens, including honey bee viruses. About 24 viruses have been reported so far. However, six of them are considered to cause severe infection which inflicts heavy losses on beekeeping. The aim of this study is to detect six honey bee viruses: deformed wing virus (DWV), acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), sacbrood virus (SBV), kashmir bee virus (KBV), and black queen cell virus (BQCV) by a Reverse transcription polymerase chain reaction (RT-PCR). A total of 50 adult honey bee samples were obtained from apiaries situated in three different parts of the country (South, North and West Bulgaria).The results showed the highest prevalence of DWV (10/20 %), followed by SBV (6/12 %) and ABPV (2/4%), and one case of BQCV. A comparison with homology sequences available in GenBank was performed by phylogenetic analysis, and phylogenetic relationships were discussed in the context of newly described genotypes in the uninvestigated South Eastern region of Europe.In conclusion, the present study has been the first to provide sequencing data and phylogenetics analyses of some honey bee viruses in Bulgaria.