Xylose fermentation to ethanol by new Galactomyces geotrichum and Candida akabanensis strains

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1 Graduate Program of Biofuels, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
2 Science and Technology Institute, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
3 Department of Pharmacy, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
4 Department of Biology, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil
5 Department of Basic Sciences, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil
DOI
10.7287/peerj.preprints.26574v1
Published
Accepted
Subject Areas
Biotechnology, Microbiology, Food, Water and Energy Nexus
Keywords
alcoholic fermentation, yeast, hemicellulose, bioethanol, pentoses
Copyright
© 2018 Valinhas et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Valinhas RV, Pantoja LA, Maia ACF, Miguel MGCP, Vanzela APFC, Nelson DL, Santos AS. 2018. Xylose fermentation to ethanol by new Galactomyces geotrichum and Candida akabanensis strains. PeerJ Preprints 6:e26574v1

Abstract

The conversion of pentoses into ethanol remains a challenge and could increase the supply of second-generation biofuels. This study sought to isolate naturally occurring yeasts from plant biomass and determine their capabilities for transforming xylose into ethanol. Three yeast strains with the ability to ferment xylose were isolated from pepper, tomato and sugarcane bagasse. The strains selected were characterized by morphological and auxanographic assays, and they were identified by homology analysis of 5.8S and 26S ribosomal RNA gene sequences. The identities of two lineages of microrganism were associated with Galactomyces geotrichum, and the other was associated with Candida akabanensis. Fermentative processes were conducted with liquid media containing only xylose as the carbon source. YP/S values for the production of ethanol ranging between 0.29 and 0.35 g g-1 were observed under non-optimized conditions.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Raw data used to Figure 3 and 4 construction

DOI: 10.7287/peerj.preprints.26574v1/supp-1